Soil waterlogging is a major environmental stress that suppresses the growth and productivity of rapeseed (Brassica napus L.). Natural genetic variations in waterlogging tolerance (WT) were observed but no QTL mapping has been done for WT related traits in rapeseed. In this study, QTL associated with three WT related traits including relative root length (RRL), relative hypocotyl length (RHL) and relative fresh weight (RFW) were dissected using a set of reciprocal introgression lines (ILs) derived from the cross GH01×ZS9, which showed significant difference in WT. Genotyping-by-sequencing (GBS) of the populations were performed, totally 1 468 and 1 450 binned SNPs were identified for GIL (GH01 as the recurrent parent) and ZIL (ZS9 as the recurrent parent) population, respectively. A total of 66 distinct QTLs for WT at the seedling establishment stage including 31 for RRL, 17 for RHL and 18 for RFW were detected. Among the 66 QTLs, 20 (29.4%) QTLs were detected in both genetic backgrounds and then they were integrated into six QTL clusters, which can be targeted in rapeseed breeding for improvement of WT through marker-assisted selection (MAS). Based on the physical positions of SNPs and the functional annotation of the Arabidopsis thaliana genome, 56 genes within the six QTL cluster regions were selected as preliminary candidate genes, then the resequencing and transcriptome information about parents were applied to narrow the extent of candidate genes. Twelve genes were determined as candidates for the six QTL clusters, some of them involved in RNA/protein degradation, most of them involved in oxidation-reduction process. These findings provided genetic resources, candidate genes to address the urgent demand of improving WT in rapeseed breeding.

土壤淹水是一种主要的环境压力，它抑制了油菜籽（甘蓝型油菜）的生长和生产力。L.）。观察到了渍水耐性（WT）的自然遗传变异，但尚未对油菜的WT相关性状进行QTL定位。在这项研究中，使用一组来自十字GH01×的互渗性系（ILs），对与三个WT相关性状相关的QTL进行了解剖，包括相对根长（RRL），相对下胚轴长度（RHL）和相对鲜重（RFW）。 ZS9，在WT中显示出显着差异。进行了测序的基因分型（GBS），分别针对GIL（GH01为轮回亲本）和ZIL（ZS9为轮回亲本）群体分别鉴定了1 468和1 450装仓的SNP。在苗建立阶段共检测到66个不同的WT QTL，包括RRL 31个，RHL 17个和RFW 18个。在66个QTL中，有20个（29。在两个遗传背景中均检测到4％的QTL，然后将它们整合到6个QTL簇中，可将其用于油菜育种，以通过标记辅助选择（MAS）改善野生型。基于单核苷酸多态性的物理位置和功能注释选择拟南芥基因组，在六个QTL簇区域内的56个基因作为初步候选基因，然后应用有关亲本的重测序和转录组信息来缩小候选基因的范围。确定了十二个基因作为六个QTL簇的候选基因，其中一些与RNA /蛋白质降解有关，其中大多数与氧化还原过程有关。这些发现提供了遗传资源，候选基因，以解决提高菜籽育种中WT的迫切需求。