The present study addresses the immobilization of the GOX enzyme into a conventional polyvinyl alcohol (PVOH) membrane and a multi-layer membrane system (PCL/PVOH/PCL), both synthetized through electrospinning technique. More specifically, the enzyme was encapsulated inside a PVOH hydrophilic membrane, which was in turn protected by two hydrophobic polycaprolactone (PCL) membranes (multilayer system). The manufactured systems were characterized through IR spectroscopy, enzymatic kinetics and mechanical properties. Moreover, the morphology of the fibers composing the membranes was studied by Scanning Electronic Microscopy (SEM) and image analysis. Finally, the enzyme activity of the systems was in-vitro bacterial cultures using E. coli. The results obtained indicate that the addition of the GOX enzyme involves a slight reduction of the mechanical properties (maximum strain at break and maximum stress), which is associated with an increase of the polydispersity of the generated fibers. However, the mats showed antibacterial properties, which were evidenced by the inhibition of E. coli growth. These results indicate that this technique is suitable for the immobilization of enzymes with potential use in the active packaging industry.

本研究致力于将GOX酶固定在常规的聚乙烯醇（PVOH）膜和多层膜系统（PCL / PVOH / PCL）中，两者均通过静电纺丝技术合成。更具体地说，将酶包封在PVOH亲水膜内，该膜依次被两个疏水性聚己内酯（PCL）膜（多层系统）保护。所制造的系统通过红外光谱，酶动力学和机械性能进行了表征。此外，通过扫描电子显微镜（SEM）和图像分析研究了组成膜的纤维的形态。最后，该系统的酶活性是使用大肠杆菌进行的体外细菌培养。获得的结果表明，GOX酶的加入会导致机械性能的轻微降低（断裂时的最大应变和最大应力），这与所产生纤维的多分散性增加有关。但是，垫子具有抗菌性能，这可以通过抑制大肠杆菌的生长来证明。这些结果表明，该技术适用于酶的固定化，在活性包装工业中具有潜在用途。