Green synthesis of metal-encased nutraceutical nano-hybrids has been a target for research over the last few years. In the present investigation, we have reported temperature dependent facile synthesis of silver nanoparticles using FDA approved c phycocyanin (cPC). The cPC conjugated silver nanoparticles (AgcPCNPs) were characterized by TEM, Zeta Potential, UV–vis, XPS, FTIR, and CD Spectroscopy. The temperature optimization studies suggested the synthesis of stable AgcPCNPs at 40 °C while at higher temperature system shows aggregated appearance. Molecular docking studies predicted the exclusive interaction of C, D, I, and J chains of cPC with the surface of AgNPs. Moreover, AgcPCNPs significantly (p < 0.1 %) counteract the toxic nature of AgNPs on red blood cell by measuring parameters like total RBC count, % hemolysis, % hematocrit, coagulation time, pH, electrolyte concentrations and degree of blood cell lipid peroxidation by the anti-oxidation mechanism. Skin fibroblast in vitro cell migration result suggeststhat AgcPCNPs enhanced the degree of cell movement towards the wound area. Data obtained collectively demonstrate that AgcPCNPs can be a better agent in the dermal wound healing with reduced toxicity with the bi-phasic advantage of cPC as a wound healer and Ag nano-metal as an anti-bacterial agent.

在过去几年中，金属包裹的营养保健纳米混合物的绿色合成一直是研究的目标。在目前的调查中，我们报告了使用 FDA 批准的 c 藻蓝蛋白 (cPC) 的温度依赖性银纳米粒子的简便合成。通过 TEM、Zeta 电位、UV-vis、XPS、FTIR 和 CD 光谱对 cPC 共轭银纳米颗粒 (AgcPCNPs) 进行了表征。温度优化研究表明在 40 °C 下合成稳定的 AgcPCNPs，而在更高温度下系统显示出聚合外观。分子对接研究预测了 cPC 的 C、D、I 和 J 链与 AgNPs 表面的排他性相互作用。此外，AgcPCNPs 显着 ( p< 0.1 %) 通过抗氧化机制测量总红细胞计数、溶血百分比、血细胞比容百分比、凝血时间、pH、电解质浓度和血细胞脂质过氧化程度等参数，抵消 AgNPs 对红细胞的毒性。皮肤成纤维细胞体外细胞迁移结果表明AgcPCNPs增强了细胞向伤口区域移动的程度。获得的数据共同表明，AgcPCNPs 可以成为皮肤伤口愈合的更好药剂，同时具有 cPC 作为伤口愈合剂和 Ag 纳米金属作为抗菌剂的双相优势，可降低毒性。