Abstract —The activities of isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, malate dehydrogenase, and NADH:ubiquinone reductase in the mitochondrial fraction from the liver tissue of rats fed diets with different sucrose and protein contents have been measured. In animals that received a high-sucrose diet, isocitrate dehydrogenase and malate dehydrogenase activities tend to decline and α-ketoglutarate dehydrogenase activity decreased by a factor of 2.5. Interestingly, the NADH:ubiquinone reductase activity remains at the control level in animals that are fed the high-sucrose diet. In the mitochondrial fraction from the liver tissue of rats that receive a high-sucrose–low-protein diet, the activities of NAD + -dependent dehydrogenases of the Krebs cycle decrease considerably, and the activity of NADH:ubiquinone reductase decreased as well, being more than 2.5 times lower than in the control. This may be one of the steps in the mechanism by which energy generated in the cell is regulated when the sucrose : protein ratio in the diet is imbalanced. These results can be used to develop a strategy for the correction of metabolic disturbances associated with nutrient contents in the diet.

中文翻译：

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不同营养成分饮食大鼠肝细胞能量代谢特征

摘要 — 已经测量了喂食不同蔗糖和蛋白质含量饮食的大鼠肝脏组织线粒体部分中异柠檬酸脱氢酶、α-酮戊二酸脱氢酶、苹果酸脱氢酶和 NADH：泛醌还原酶的活性。在接受高蔗糖饮食的动物中，异柠檬酸脱氢酶和苹果酸脱氢酶活性趋于下降，α-酮戊二酸脱氢酶活性下降 2.5 倍。有趣的是，在喂食高蔗糖饮食的动物中，NADH：泛醌还原酶活性保持在对照水平。在接受高蔗糖-低蛋白饮食的大鼠肝脏组织的线粒体部分中，克雷布斯循环的 NAD + 依赖性脱氢酶的活性显着降低，并且 NADH：泛醌还原酶的活性也降低，比对照低 2.5 倍以上。这可能是当饮食中的蔗糖：蛋白质比例不平衡时调节细胞中产生的能量的机制中的步骤之一。这些结果可用于制定纠正与饮食中营养成分相关的代谢紊乱的策略。