Abstract —The efficiency of phosphorylation in samples of isolated rat liver mitochondria in incubation media with variable isotopic composition at deuterium contents of 6, 60, 90, 120, 150 ppm, as well as 0.1, 1 and 2%, was studied. In media with a low deuterium content (up to 60 ppm), a monotonic decrease in the ADP/O ratio was observed, while at 6 ppm its value was the same as at 60 ppm. The generation of superoxide anion by complex I, II, and III of the mitochondrial respiratory chain was also investigated. For all studied modes of mitochondrial functioning using respiratory chain inhibitors, the generation of superoxide anion was independent of the deuterium concentration in the incubation medium.

中文翻译：

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贫氘水中线粒体的功能活性

摘要 —研究了在氘含量为 6、60、90、120、150 ppm 以及 0.1、1 和 2% 的同位素组成可变的孵育培养基中分离大鼠肝线粒体样品的磷酸化效率。在低氘含量（高达 60 ppm）的介质中，观察到 ADP/O 比值单调下降，而在 6 ppm 时其值与 60 ppm 时相同。还研究了线粒体呼吸链复合物 I、II 和 III 产生的超氧阴离子。对于所有使用呼吸链抑制剂研究的线粒体功能模式，超氧阴离子的产生与培养介质中的氘浓度无关。