Fusarium wilt of tomato caused by the pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is one of the most devastating soilborne diseases of tomato. To evaluate whether microbial community composition associated with Fol-infected tomato is different from healthy tomato, we analyzed the tomato-associated microbes in both healthy and Fol-infected tomato plants at both the taxonomic and functional levels; both bacterial and fungal communities have been characterized from bulk soil, rhizosphere, rhizoplane, and endosphere of tomatoes using metabarcoding and metagenomics approaches. The microbial community (bacteria and fungi) composition of healthy tomato was significantly different from that of diseased tomato, despite similar soil physicochemical characteristics. Both fungal and bacterial diversities were significantly higher in the tomato plants that remained healthy than in those that became diseased; microbial diversities were also negatively correlated with the concentration of Fol pathogen. Network analysis revealed the microbial community of healthy tomato formed a larger and more complex network than that of diseased tomato, probably providing a more stable community beneficial to plant health. Our findings also suggested that healthy tomato contained significantly greater microbial consortia, including some well-known biocontrol agents (BCAs), and enriched more functional genes than diseased tomato. The microbial taxa enriched in healthy tomato plants are recognized as potential suppressors of Fol pathogen invasion.

由病原体尖孢镰刀菌f引起的番茄枯萎病。sp. lycopersici ( Fol ) 是番茄最具破坏性的土传病害之一。为了评估与Fol感染的番茄相关的微生物群落组成是否与健康番茄不同，我们分析了健康和Fol 中与番茄相关的微生物。- 在分类和功能水平上受感染的番茄植株；使用宏条形码和宏基因组学方法从番茄的大块土壤、根际、根际和内圈中表征了细菌和真菌群落。尽管土壤理化特征相似，但健康番茄的微生物群落（细菌和真菌）组成与患病番茄显着不同。保持健康的番茄植株的真菌和细菌多样性明显高于患病的番茄植株；微生物多样性也与Fol的浓度呈负相关病原。网络分析表明，健康番茄的微生物群落形成了一个比患病番茄更大、更复杂的网络，可能为植物健康提供了一个更稳定的群落。我们的研究结果还表明，健康番茄含有明显更多的微生物群落，包括一些众所周知的生物防治剂 (BCA)，并且比患病番茄富含更多的功能基因。富含健康番茄植物的微生物分类群被认为是Fol病原体入侵的潜在抑制因子。