The gut microbiome, which is symbiotic within the human body, assists in human digestion. It plays significant roles in identifying intestinal disease as well as in maintaining a healthy body with functional immune and metabolic activities. To confirm the consistency of fecal intestinal microbial research, it is necessary to study the changes in intestinal microbial flora according to the fecal collection solution and storage period. We collected fecal samples from three healthy Korean adults. To examine the efficacy of fecal collection solution, we used NBgene-Gut, OMNIgene-Gut, 70% ethanol (Ethanol-70%), and RNAlater. The samples were stored for up to two months at room temperature using three different methods, and we observed changes in microbial communities over time. We analyzed clusters of changes in the microbial flora by observing fecal stock solutions and metagenome sequencing performed over time. In particular, we confirmed the profiling of alpha and beta diversity and microbial classification according to the differences in intestinal environment among individuals. We also confirmed that the microbial profile remained stable for two months and that the microbial profile did not change significantly over time. In addition, our results suggest the possibility of verifying microbial profiling even for long-term storage of a single sample. In conclusion, collecting fecal samples using a stock solution rather than freezing feces seems to be relatively reproducible and stable for GUT metagenome analysis. Therefore, stock solution tubes in intestinal microbial research can be used without problems.

中文翻译：

---

粪便防腐剂和原液在室温下用于肠道微生物组储存的性能比较。

肠道微生物组在人体中是共生的，有助于人体消化。它在识别肠道疾病以及维持具有功能性免疫和代谢活性的健康身体方面发挥着重要作用。为了确认粪便肠道微生物研究的一致性，有必要根据粪便收集液和保存时间来研究肠道微生物菌群的变化。我们从三名健康的韩国成年人那里收集了粪便样本。为了检查粪便收集溶液的功效，我们使用了NBgene-Gut，OMNIgene-Gut，70％的乙醇（乙醇-70％）和RNAlater。使用三种不同的方法将样品在室温下保存长达两个月，我们观察了微生物群落随时间的变化。我们通过观察粪便原液和随时间推移进行的基因组测序来分析微生物菌群变化的簇。特别是，我们根据个体之间肠道环境的差异确认了α和β多样性以及微生物分类的概况。我们还确认，微生物谱在两个月内保持稳定，并且微生物谱没有随时间变化显着。此外，我们的结果表明，即使对于单个样品的长期储存，也有可能验证微生物谱。总之，使用储液而不是冷冻粪便收集粪便样本对于GUT的基因组分析似乎相对可重复且稳定。因此，可以毫无问题地使用肠道微生物研究中的储备溶液管。