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Identification and phylogenetic analysis of Burkholderia cepacia complex bacteria isolated from rot of onion bulbs in Tohoku region of Japan
Journal of General Plant Pathology ( IF 1.2 ) Pub Date : 2020-06-25 , DOI: 10.1007/s10327-020-00937-z
Mizue Tsuji , Ikuo Kadota

Burkholderia cepacia complex (Bcc) bacterial isolates from onion bulbs with rot in the Tohoku region of Japan were identified using a pathogenicity test, phenotypic characterization, restriction fragment length polymorphism (RFLP) analysis, and phylogenetic analyses based on 16S rRNA and housekeeping genes. Onion isolates caused a rot on onion bulbs similar to the field rot. Phenotypic properties of onion isolates were consistent with those of Bcc strains reported previously. The RFLP analysis using recA gene PCR amplicon from the onion isolates showed five distinct banding patterns. On the basis of phylogenetic analyses using 16S rRNA gene and housekeeping genes gyrB, rpoD, and recA, onion isolates were identified as B. cepacia, B. cenocepacia, B. ambifaria, and B. pyrrocinia, members of Bcc. In the phylogenetic tree constructed using the housekeeping gene sequences, onion isolates and reference strains producing a purple nondiffusible pigment formed a group in the B. cepacia branch. B. ambifaria and B. pyrrocinia caused rot symptoms on onion the same as those caused by B. cepacia and B. cenocepacia strains.

中文翻译:

日本东北地区洋葱鳞茎腐烂的洋葱伯克霍尔德菌复合细菌的鉴定和系统发育分析

基于致病性测试,表型表征,限制性片段长度多态性(RFLP)分析以及基于16S rRNA和管家基因的系统发育分析,确定了日本东北地区洋葱鳞茎腐烂的洋葱伯克霍尔德菌种(Bcc)细菌分离物。洋葱分离物导致洋葱鳞茎腐烂,类似于田间腐烂。洋葱分离物的表型特性与先前报道的Bcc菌株一致。使用来自洋葱分离株的recA基因PCR扩增子进行的RFLP分析显示了五个不同的条带模式。根据使用16S rRNA基因和管家基因gyrBrpoDrecA的系统发育分析,洋葱分离株被鉴定为。洋葱伯克霍尔德菌新洋葱伯克霍尔德杆菌B. ambifariaB. pyrrocinia,密件抄送的成员。在使用管家基因序列构建的系统树中,洋葱分离株和产生紫色不可扩散色素的参考菌株在洋葱伯克霍尔德菌分支中形成一个基团。B. ambifariaB. pyrrocinia引起洋葱上的那些相同引起的腐烂症状洋葱伯克霍尔德菌新洋葱伯克霍尔德杆菌菌株。
更新日期:2020-06-25
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