The breast cancer susceptibility protein 1 (BRCA1) plays a central role in the suppression of human breast and ovarian cancer. Germ line mutations of the BRCA1 gene are responsible for the hereditary breast and ovarian cancer (HBOC) syndrome. Here were report ¹H, ¹³C, and ¹⁵N resonance assignments for the intrinsically disordered BRCA1 fragment 219–504, which contains important interaction sites for the proto-oncogenic transcription factor MYC as well as for p53. A nuclear magnetic resonance assignment was achieved at 18.8 T magnetic field strength using a 5D HN(CA)CONH experiment and its associated 4D H(NCA)CONH and 4D (H)N(CA)CONH experiments. ¹³C^α and ¹³C^β assignments were obtained using a 5D HabCabCONH experiment. With this strategy, 90% of ¹H/¹⁵N backbone pairs could be assigned. Similarly, 264 C’ resonances were assigned corresponding to 86% of the total number of C’ atoms. In addition, 252 C^β resonances (i.e. 85%) were assigned, together with 461 attached H^β nuclei, as well as 264 (i.e. 86%) C^α resonances, together with 275 attached H^α nuclei.

中文翻译：

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BRCA1 片段 219-504 的 1H、13C 和 15N 骨架共振分配。

乳腺癌易感蛋白 1 (BRCA1) 在抑制人类乳腺癌和卵巢癌中起着核心作用。BRCA1基因的种系突变是导致遗传性乳腺癌和卵巢癌 (HBOC) 综合征的原因。这里报告了固有无序 BRCA1 片段 219-504 的¹ H、¹³ C 和¹⁵ N 共振分配，其中包含原癌转录因子 MYC 和 p53 的重要相互作用位点。使用 5D HN(CA)CONH 实验及其相关的 4D H(NCA)CONH 和 4D (H)N(CA)CONH 实验在 18.8 T 磁场强度下实现了核磁共振分配。¹³ C ^α和¹³C ^β分配是使用 5D HabCabCONH 实验获得的。使用此策略，可以分配¹ H/ ¹⁵ N 主干对中的90% 。类似地，分配了 264 个 C' 共振，对应于 C' 原子总数的 86%。此外，分配了252 个 C ^β共振（即 85%）以及 461 个连接的 H ^β核，以及 264 个（即 86%）C ^α共振以及 275 个连接的 H ^α核。