ABSTRACT

A magnetic electrospun nanofibre was prepared and applied to load and release bovine serum albumin (BSA). Fe₃O₄ magnetic nanoparticles were prepared by chemical co-precipitation of ferric and ferrous salts and coated with polyvinyl alcohol/collagen electrospun nanofibre. Tripolyphosphate (TPP) and glutaraldehyde were used to stabilise nanoparticles within the electrospun nanofibres. It was found from scanning electron microscopy graphs that TPP is more suitable than glutaraldehyde and a network of relatively tall and perfectly uniform fibres with smooth surface can be produced and magnetic nanoparticles in their smallest size are distributed all over the fibres network without any agglomeration. BSA protein was loaded in magnetic nanofibres and its releasing under magnetic field and in the absence of magnetic field was studied using the Bradford assay method. The magnetic nanofibres completely changed the structure and formed pores and cracks under the magnetic field and then released BSA after 3 h, about 12 times higher than its releasing in the absence of magnetic field.

摘要

制备了磁性电纺纳米纤维并将其用于加载和释放牛血清白蛋白（BSA）。Fe ₃ O ₄通过铁盐和亚铁盐的化学共沉淀制备磁性纳米粒子，并用聚乙烯醇/胶原电纺纳米纤维包覆。三聚磷酸盐（TPP）和戊二醛用于稳定电纺纳米纤维内的纳米颗粒。从扫描电镜图中发现，TPP比戊二醛更适合，可以生产出相对较高且完全均匀且表面光滑的纤维网络，最小尺寸的磁性纳米粒子分布在整个纤维网络中，没有任何团聚。将 BSA 蛋白加载到磁性纳米纤维中，并使用 Bradford 分析方法研究其在磁场和无磁场下的释放。