The tortoiseshell coat colour is characteristic to female cats, and its occurrence in tomcats is very rare and associated with chromosome abnormalities (additional copy of X chromosome). The aim of this study was identification of the genetic basis of a case of tortoiseshell colour in a fertile Maine coon tomcat. Cytogenetic and molecular genetic studies were carried out with painting molecular probes (WCPP) specific to the X and Y sex chromosomes as well as a DNA microsatellite panel for the parentage verification of cats. Cytogenetic analysis revealed only a single set of sex chromosomes typical for male – 38,XY. The results of the microsatellite polymorphism obtained from DNA showed three alleles in locus FCA201 and four alleles in loci FCA149 and FCA441 in different tissues (blood, hair roots and testicles). Based on these results, the case was diagnosed as a true chimerism 38,XY/38,XY. To the best of our knowledge, this is the first case of a 38,XY/38,XY chimera diagnosed in cats, confirmed by genetic analysis.

中文翻译：

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真正嵌合的可育雄性cat猫38，XY / 38，XY。

female颜色是雌性猫的特征，它在雄猫中的出现非常罕见，并且与染色体异常（X染色体的其他副本）有关。这项研究的目的是鉴定可育的缅因州浣熊中a色的遗传基础。细胞遗传学和分子遗传学研究是通过对X和Y性染色体具有特异性的绘画分子探针（WCPP）以及用于猫的亲缘鉴定的DNA微卫星面板进行的。细胞遗传学分析显示，只有一组典型的男性性染色体-38，XY。从DNA获得的微卫星多态性的结果显示在3个等位基因基因座FCA201和四个等位基因基因座FCA149和FCA441位于不同组织（血液，发根和睾丸）中。根据这些结果，该病例被诊断为真正的嵌合体38，XY / 38，XY。据我们所知，这是在猫中诊断出的38，XY / 38，XY嵌合体的第一例，经基因分析证实。