In article number 2000364 by Yanyan Wang, Zefang Wang, and co‐workers, an ultrasensitive and rapid thrombin biosensor is developed based on rationally designed trifunctional protein HTs, comprising three functional units, including a far‐red fluorescent protein smURFP, hydrophobin HGFI, and a thrombin cleavage site (TCS). smURFP is used as a detection signal to eliminate any interference from the autofluorescence of sample matrix to increase detection sensitivity. HGFI serves as an adhesive unit to allow rapid immobilization of HTs on a multiwall plate. The TCS linking HGFI and smURFP functions as a sensing element to recognize and detect thrombin. As a result, the intensity of the fluorescent signal on the surface of multiwall plates changes depending on the concentration of thrombin. The sensing mechanism of this biosensor relies on the ability of active thrombin in samples to specifically recognize and hydrolyze its cleavage site in the fusion protein, resulting in a subsequent decrease in the fluorescence intensity of the sample.

中文翻译：

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凝血酶生物传感器：基于合理设计的三功能蛋白的快速，超灵敏的凝血酶生物传感器（Adv。Healthcare Mater。12/2020）

在王艳艳，王泽芳及其同事的文章2000364中，基于合理设计的三功能蛋白HTs开发了一种超灵敏，快速的凝血酶生物传感器，该三功能蛋白HTs包含三个功能单元，包括远红色荧光蛋白smURFP，疏水蛋白HGFI和凝血酶裂解位点（TCS）。smURFP用作检测信号，可消除样品基质自发荧光的任何干扰，从而提高检测灵敏度。HGFI用作粘合单元，可将HT快速固定在多层板上。连接HGFI和smURFP的TCS充当识别和检测凝血酶的传感元件。结果，多壁板表面上的荧光信号的强度根据凝血酶的浓度而变化。