Glyphosate (GLY) is an herbicidal active ingredient and both in vitro and in vivo studies suggest that GLY alters ovarian function. To determine if a chronic GLY exposure model affected steroidogenesis or folliculogenesis in vivo, postnatal day 42 C57BL6 female mice were orally delivered vehicle control (saline) or GLY (2 mg/Kg) from a pipette tip five days per week for either five or ten weeks. Mice were euthanized at the pro-estrus stage of the estrous cycle. GLY exposure did not impact body weight gain, organ weights, or healthy follicle numbers. In addition, GLY exposure did not affect abundance of ovarian mRNA encoding kit ligand (Kitlg), KIT proto-oncogene receptor tyrosine kinase (c-Kit), insulin receptor (Insr), insulin receptor substrate (Irs1 or Irs2) and protein thymoma viral proto-oncogene 1 (AKT) or phosphorylated AKT. Ovarian mRNA or protein abundance of Star, 3β-hydroxysteroid dehydrogenase (Hsd3b1), Cyp11a1 or Cyp19a were also not altered by GLY. Circulating 17β-estradiol and progesterone concentration were unaffected by GLY exposure. In conclusion, chronic GLY exposure for five or ten weeks did not affect the ovarian endpoints examined herein.

草甘膦 ( GLY ) 是一种除草活性成分，体外和体内研究均表明 GLY 会改变卵巢功能。为了确定慢性 GLY 暴露模型是否影响体内类固醇生成或卵泡生成，出生后第 42 天 C57BL6 雌性小鼠每周 5 天从移液管尖端口服载体对照（盐水）或 GLY（2 mg/Kg），持续 5 天或 10 天周。在发情周期的前发情阶段对小鼠实施安乐死。GLY 暴露不会影响体重增加、器官重量或健康卵泡数量。此外，GLY 暴露不影响卵巢 mRNA 编码试剂盒配体(Kitlg)、KIT 原癌基因受体酪氨酸激酶 (c- Kit)的丰度、胰岛素受体 ( Insr)、胰岛素受体底物 ( Irs1或Irs2 )和蛋白质胸腺瘤病毒原癌基因 1 (AKT) 或磷酸化 AKT。Star、3β-羟基类固醇脱氢酶 ( Hsd3b1)、Cyp11a1或Cyp19a的卵巢 mRNA 或蛋白质丰度也没有被 GLY 改变。循环 17β-雌二醇和孕酮浓度不受 GLY 暴露的影响。总之，五周或十周的慢性 GLY 暴露不影响本文检查的卵巢终点。