Sodium pheophorbide a (SPA) is a new alternative fungicide with low toxicity and high efficiency, which has high fungicidal activity against Pestalotiopsis neglecta, a pathogen that causes black spot needle blight of Pinus sylvestris var. mongolica. To utilize SPA for plant disease control, understanding its antifungal mechanism is essential. Six cDNA libraries were constructed from 3 d-old P. neglecta mycelia (three SPA-infected and three untreated groups) and 29,850 expressed genes were obtained by Illumina HiSeq4000 sequencing. Compared with controls, 3268 differentially expressed genes (DEGs) were identified in SPA-treated groups, including 1879 upregulated and 1389 downregulated genes. Most DEGs were involved in the metabolism of amino acids, carbohydrates, and lipids, as well as cell structure and genetic information processing. These findings were further confirmed by decreased conductivity, RNA and protein content, and activities of nicotinamide adenine dinucleotide-dependent malate dehydrogenase, citrate synthase, isocitrate dehydrogenase, and succinate dehydrogenase. Moreover, qRT-PCR verified the reliability of the transcriptome results. After treatment with SPA at different concentrations for 60 min, the expressions of three cell wall degrading enzyme-related genes (PnEG, PnBG, and PnPG) were all suppressed. Overall, this study provided insights into the molecular mechanisms through which SPA inhibits P. neglecta, increasing the possibility of developing SPA into an effective fungicide in the future.

中文翻译：

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对脱镁叶绿素 a 反应的 Pestalotiopsis una 的转录组和细胞壁降解酶相关基因分析

脱镁叶绿素a(SPA)是一种低毒高效的新型替代杀菌剂，对引起樟子松黑斑针枯病的病原菌Pestalotiopsis noticea具有较高的杀菌活性。蒙古。要利用 SPA 控制植物病害，了解其抗真菌机制至关重要。6 个 cDNA 文库由 3 d 龄的小球藻菌丝体（三个 SPA 感染组和三个未处理组）构建，并通过 Illumina HiSeq4000 测序获得 29,850 个表达基因。与对照组相比，SPA处理组共鉴定出3268个差异表达基因（DEGs），包括1879个上调基因和1389个下调基因。大多数DEGs参与氨基酸、碳水化合物和脂质的代谢，以及细胞结构和遗传信息处理。这些发现通过降低的电导率、RNA 和蛋白质含量以及烟酰胺腺嘌呤二核苷酸依赖性苹果酸脱氢酶、柠檬酸合酶、异柠檬酸脱氢酶和琥珀酸脱氢酶的活性得到进一步证实。此外，qRT-PCR 验证了转录组结果的可靠性。用不同浓度的SPA处理60分钟后，三种细胞壁降解酶相关基因（PnEG、PnBG和PnPG）的表达均受到抑制。总体而言，这项研究提供了对 SPA 抑制 P. ignorea 的分子机制的见解，增加了未来将 SPA 开发为有效杀菌剂的可能性。柠檬酸合酶、异柠檬酸脱氢酶和琥珀酸脱氢酶。此外，qRT-PCR 验证了转录组结果的可靠性。用不同浓度的SPA处理60分钟后，三种细胞壁降解酶相关基因（PnEG、PnBG和PnPG）的表达均受到抑制。总体而言，这项研究提供了对 SPA 抑制 P. ignorea 的分子机制的见解，增加了未来将 SPA 开发为有效杀菌剂的可能性。柠檬酸合酶、异柠檬酸脱氢酶和琥珀酸脱氢酶。此外，qRT-PCR 验证了转录组结果的可靠性。用不同浓度的SPA处理60分钟后，三种细胞壁降解酶相关基因（PnEG、PnBG和PnPG）的表达均受到抑制。总体而言，这项研究提供了对 SPA 抑制 P. ignorea 的分子机制的见解，增加了未来将 SPA 开发为有效杀菌剂的可能性。