Since humans are exposed to different mycotoxins through daily intake, there is increasing concern about the adverse effects of the interactions between them. Cytotoxicity of sterigmatocystin (STE) and nivalenol (NIV) alone and in combination in human hepatocarcinoma (HepG2) cells was evaluated by MTT assay. Furthermore, ROS production and alteration of ΔΨm as mechanisms of action were assessed. Cells were treated with concentrations ranging from 0.15 to 5 μM for NIV and from 0.78 to 50 μM for STE individually and in binary combinations. The combination ratio between the mixture STE + NIV was 10:1. The IC₅₀ values of NIV ranged from 0.96 to 0.66 μM, whereas no IC₅₀ values were obtained for STE at any time tested. For the combinations studied, synergistic, antagonistic and addictive effects were obtained with the two type of analyses performed, the isobologram analysis and the Combenefit method. No relevant effects on ROS and ΔΨm were observed. In conclusion, predictive models based on combination data could help to better understand the interactions between mycotoxins and their implications in food safety assessment. However, a further analysis of the molecular mechanism underlying these interactive effects is required.

由于人类通过每日摄入而暴露于不同的霉菌毒素，因此人们越来越关注它们之间相互作用的不利影响。通过MTT测定评估了单独的和组合的人葡萄球菌毒素（STE）和nivalenol（NIV）对人肝癌（HepG2）细胞的细胞毒性。此外，评估了ROS的产生和ΔΨm的改变作为作用机理。NIV的浓度范围为0.15至5μM，STE的浓度范围为0.78至50μM，分别或以二元组合处理细胞。STE + NIV混合物的混合比为10：1。NIV的IC ₅₀值为0.96至0.66μM，而没有IC ₅₀在任何测试时间均可获得STE的值。对于所研究的组合，通过进行等效线图分析和Combenefit方法这两种分析，获得了协同，拮抗和成瘾作用。没有观察到对ROS和Δm的相关影响。总之，基于组合数据的预测模型可以帮助更好地了解霉菌毒素之间的相互作用及其对食品安全评估的影响。但是，需要进一步分析这些相互作用效应的分子机制。