The synthesis of discrete nanoparticle self-assemblies has challenged the nanotechnology field for decades and has limited the study of nanoparticle interactions and the advance of nanomachinery. Here, we describe the preparation of discrete nanoparticle dimers from spherical gold nanoparticles and gold nanorods with stimuli-responsive optical properties as a result of site-specific DNA functionalization with an active triple helix motif. We present discrete assemblies using anisotropic nanoparticles that dynamically respond to changes in pH. Our approach to creating building blocks for nanoparticle self-assembly capable of creating discrete structures may allow for the future study of nanoparticle-nanoparticle interactions, plasmonic sensors, and nanoparticle-DNA hybrid nanomachinery.

离散的纳米粒子自组装体的合成已经挑战了纳米技术领域数十年，并且限制了纳米粒子相互作用的研究以及纳米机械的发展。在这里，我们描述了由球形金纳米颗粒和金纳米棒制备的离散纳米颗粒二聚体，由于具有活性三螺旋基序的位点特异性DNA功能化，具有刺激响应的光学特性。我们提出了使用各向异性纳米粒子的离散组件，该纳米粒子可以动态响应pH的变化。我们为能够创建离散结构的纳米粒子自组装创建构造块的方法，可能允许对纳米粒子与纳米粒子的相互作用，等离子体传感器以及纳米粒子与DNA混合的纳米机械的进一步研究。