This paper aims to determine the denitrification strategies of strain YSF15 in carbon scarcity condition from novel view of organic nitrogen, soluble microbial products (SMP) and extracellular polymeric substances (EPS). The batch tests demonstrated that strain YSF15 could achieve complete denitrification at C/N of 3.0. The conversion ratio of nitrogen gas accounted for 89.03%, 85.29% and 82.95% among total nitrogen in C/N systems from 3.0 to 5.0, respectively, indicating denitrification instead of assimilation was the major contribution to nitrogen removal. C/N could affect composition and content of organic nitrogen, SMP and EPS. The biodegradability of EPS was better than SMP, whereas polysaccharide (PS) likely correlated with nitrogen removal, predating the protein (PN). These results implied high biodegradability of EPS and more electron donors for denitrification both improved denitrification capacity of strain YSF15, which revealed the potential contribution of bacterium with production of biodegradable SMP or EPS in biological treatment process.

本文旨在从有机氮，可溶性微生物产物（SMP）和细胞外聚合物（EPS）的新观点出发，确定碳缺乏条件下菌株YSF15的反硝化策略。批量测试表明，菌株YSF15在C / N为3.0时可以完全脱氮。在C / N系统中，氮气在总氮中的转化率从3.0到5.0分别占89.03％，85.29％和82.95％，这表明脱氮而不是同化是脱氮的主要作用。C / N会影响有机氮，SMP和EPS的组成和含量。EPS的生物降解性优于SMP，而多糖（PS）可能与脱氮相关，比蛋白质（PN）早。