Fishmeal is an important source of protein in the fish diet, but its supply is unstable due to environmental pollution and overfishing of anchovy and herring. In addition, the disease outbreak of fish has been increased, and the abuse of antibiotics and vaccines has been becoming a problem for residual substances. Therefore, studies that address diets with reduced fishmeal content and additives that can increase efficiency and replace antibiotics are needed. Hence, in this study, the probiotics bacteria Bacillus sp. SJ-10 (BSJ-10) and Lactobacillus plantarum (LP) were heat-killed (HK) used as additives in a low-fishmeal diet. The two bacteria with a 45 % fishmeal diet were added at a concentration of 3.34 × 10⁸ CFU/mL, and both of probiotics bacteria were mixed and added to the same concentration in order to produce the diet for HK BSJ-10 (HKBSJ-10), HK LP (HK-LP), and MIX (HKBSJ-10 + HK-LP) groups. We raised four replicates of juvenile olive flounder for 8 weeks. In the HKBSJ-10 group, the plasma insulin, GH, and IGF-I level were higher than the other groups (P < 0.05). Similarly, intramuscular IGF-I activity and signaling pathway were higher in HKBSJ-10 (P < 0.05). The innate immunity lysosome activity was higher in the HKBSJ-10 and MIX groups (P < 0.05), even cholecystokinin manifestation, intestinal wall thickness, and the density of the intestinal folds more evolved in the HKBSJ-10 group. Therefore, the HKBSJ-10 additive could be advanced in growth-related endocrine, innate immunity, and intestinal morphology, positively affecting a low-fishmeal diet for juvenile olive flounder.

鱼粉是鱼饲料中蛋白质的重要​​来源，但由于环境污染以及an鱼和鲱鱼的过度捕捞，鱼粉的供应不稳定。此外，鱼类疾病的爆发已经增加，抗生素和疫苗的滥用已成为残留物质的问题。因此，需要针对减少鱼粉含量的饮食和可以提高效率并替代抗生素的添加剂进行研究。因此，在这项研究中，益生菌细菌为芽孢杆菌。SJ-10（BSJ-10）和植物乳杆菌（LP）被热杀死（HK），用作低鱼粉饮食中的添加剂。鱼粉日粮含量为45％的两种细菌的​​添加浓度为3.34×10 ⁸CFU / mL和两种益生菌细菌混合并添加至相同浓度，以生产HK BSJ-10（HKBSJ-10），HK LP（HK-LP）和MIX（HKBSJ-10 + HK -LP）组。我们饲养了四个幼橄榄比目鱼，历时8周。HKBSJ-10组的血浆胰岛素，GH和IGF-I水平高于其他组（P <0.05）。同样，HKBSJ-10的肌内IGF-I活性和信号通路更高（P <0.05）。HKBSJ-10和MIX组的先天免疫溶酶体活性较高（P <0.05），甚至胆囊收缩素的表现，肠壁厚度和肠褶密度的变化在HKBSJ-10组中更明显。因此，HKBSJ-10添加剂可以促进生长相关的内分泌，先天免疫和肠道形态，