Abstract

Two new sesquiterpenoids, artemlavanins A (1) and B (3), together with fifteen known compounds (2 and 4−17) were isolated from the EtOH extract of Artemisia lavandulaefolia. The structures of new compounds were elucidated by extensive spectroscopic analyses (HRESIMS, 1D and 2D NMR) and ECD calculations. Compound 1 was a sesquiterpenoid lactone possessing a rearranged eudesmane skeleton; compounds 2–5, 6–8, 9 and 10–12 belonged to the eudesmane, guaiane, oppositane and farnesane sesquiterpenoids, respectively; compounds 13–17 were the phenyl derivatives with a 4-hydroxyacetophenone moiety. Twelve compounds (1–3, 5–7, 10–12, 14, 15 and 17) displayed cytotoxicity against hepatic stellate cell line LX2 (HSC-LX2) with IC₅₀ values ranging from 35.1 to 370.3 μM. Compounds 2, 7, 10–12 and 17 exhibited the stronger cytotoxicity than silybin (IC₅₀, 169.6 μM) with IC₅₀ values of 82.1, 35.1, 95.0, 83.8, 81.6 and 90.1 μM. Compound 7 as the most active one showed significant inhibition on the deposition of human collagen type I (Col I), human hyaluronic acid (HA) and human laminin (HL) with IC₅₀ values of 10.7, 24.5 and 13.3 μM.

Graphic Abstract

中文翻译：

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薰衣草中的蒿素 A 和 B 及其对肝星状细胞系 LX2 的细胞毒性。

摘要

从薰衣草的乙醇提取物中分离出两种新的倍半萜类化合物，青蒿素 A ( 1 ) 和 B ( 3 )，以及 15 种已知化合物（2和4−17 ） 。通过广泛的光谱分析（HRESIMS、一维和二维核磁共振）和 ECD 计算阐明了新化合物的结构。化合物1是具有重排桉烷骨架的倍半萜内酯；化合物2-5、6-8、9和10-12分别属于桉烷、愈创木烷、对映烷和金合欢烷倍半萜类化合物；化合物13-17是带有4-羟基苯乙酮部分的苯基衍生物。12 种化合物 ( 1–3 , 5–7 , 10–12 , 14 , 15和17 ) 对肝星状细胞系 LX2 (HSC-LX2) 显示出细胞毒性，IC ₅₀值范围为 35.1 至 370.3  μM 。化合物2 , 7、10-12和17表现出比水飞蓟宾更强的细胞毒性(IC ₅₀ , 169.6  μM )，IC ₅₀值为82.1、35.1、95.0、83.8、81.6和90.1  μM。化合物7作为最活跃的一种对水飞蓟宾显示出显着的抑制作用。人 I 型胶原蛋白 (Col I)、人透明质酸 (HA) 和人层粘连蛋白 (HL) 的沉积，IC ₅₀值为 10.7、24.5 和 13.3  μM。

图文摘要