Meloidogyne graminicola is one of the most important plant-parasitic nematodes in rice. Breeding for natural resistance and tolerance is considered one of the most economical and sustainable approaches to control this damaging pathogen. Quantitative trait loci (QTL) mapping was carried out in a recombinant inbred line (RIL) population derived from a cross between the Asian rice genotypes IR78877-208-B-1-2 and Dinorado. IR78877-208-B-1-2 is an aerobic rice genotype that is resistant and tolerant to M. graminicola. Dinorado is a traditional upland rice genotype from the Philippines that is characterized by its aroma and prized for its cooking quality. The host response of 300 F₄ lines derived from this cross was evaluated in both nematode infested and non-infested concrete beds in the dry seasons of 2013 and 2014. Five genotypes were found consistently resistant while 13 genotypes were consistently partially resistant to M. graminicola. QTL mapping for M. graminicola resistance/tolerance, yield-contributing traits, and yield revealed four main effect QTLs (qJ₂RS_2.1, qJ₂GRT_2.1, qJ₂RS_3.1, and qJ₂GRT_3.1) associated with reduced nematode reproduction on chromosomes 2 and 3 and two QTLs (qGR_3.1 and qGR_5.1) associated with reduced root galling on chromosomes 3 and 5. Twenty main effect QTLs associated with yield-contributing traits and yield were also found. Our study further identified co-locating QTLs associated with nematode resistance, yield-contributing traits, and yield on chromosomes 3 and 5. QTLs harboring candidate genes, such as OsBAK1, OsDST, OsIPT4, and DUF26, for higher grain yield and tolerance to abiotic and biotic stress, were identified. These QTLs and the RILs that are consistently resistant and tolerant to M. graminicola, and which are high-yielding in nematode-infested fields, can be utilized in breeding programs to improve the resistance and tolerance of Asian rice to this important pathogen.

根结线虫是水稻中最重要的植物寄生线虫之一。天然抗药性和耐受性育种被认为是控制这种有害病原体的最经济，最可持续的方法之一。在亚洲水稻基因型IR78877-208-B-1-2和Dinorado之间杂交的重组自交系（RIL）群体中进行了数量性状基因座（QTL）定位。IR78877-208-B-1-2是一种有氧水稻基因型，对禾本科分枝杆菌有抗性和耐受性。Dinorado是菲律宾的一种传统陆稻基因型，以其香气为特征，并以其烹饪质量而倍受赞誉。主机响应300 F ₄在2013年和2014年的干旱季节，在线虫感染和未感染的混凝土床上均评估了从该杂交得到的品系。发现有5个基因型始终具有抗性，而13个基因型始终对部分灰霉菌具有抗性。QTL定位为M.蛛抗性/耐受性，产量性状贡献，和产量显示四个主要效QTL（QJ ₂ - [R小号_2.1，QJ ₂ GRT _2.1，QJ _2个RS _3.1，和QJ ₂ GRT _{3. 1}）与2号和3号染色体上的线虫繁殖减少有关，以及两个与3号和5号染色体上的根ing减少有关的QTL（qGR _3.1和qGR _5.1）。还发现了20个与产量贡献性状和产量相关的主要效应QTL。我们的研究进一步鉴定了与线虫抗性，产量贡献性状和染色体3和5上的产量相关的共定位QTL。具有候选基因（如OsBAK1，OsDST，OsIPT4和DUF26）的QTL具有较高的谷物产量和对非生物的耐受性和生物压力，被确定。这些QTL和RIL始终对耐食性分枝杆菌具有耐受性可以在育种计划中利用线虫（在线虫感染的领域中是高产的）提高亚洲水稻对该重要病原体的抗性和耐受性。