2-Nonenal is a long-chain aliphatic aldehyde containing nine carbons and an unsaturated bond. 2-Nonenal is the primary cause of odor associated with aging, with an unpleasant greasy and grassy odor. Lysosome, mitochondria, and peroxisome are significant organelles in eukaryotic cells that contain various hydrolases that degrade biomolecules. Proteins in mitochondria and peroxisome also contain aldehyde dehydrogenase. We performed trans-2-nonenal treatment using lysosomal-related enzymes extracted from hen egg white (HEW). As trans-2-nonenal is more structurally stable than cis-2-nonenal, it was selected as the target aldehyde. HEW contains various biologically active proteins and materials such as albumin, ovotransferrin, lysosome, peroxisome, and mitochondria. Here, complementary experiments were conducted to evaluate the role of lysosomal-related enzymes in the treatment of trans-2-nonenal. The activity of lysosomal-related enzymes was confirmed via antimicrobial test against E. coli. HPLC analysis was used to determine the reduction of trans-2-nonenal. The trans-2-nonenal treatment depended on the reaction time and enzyme concentration. Materials considered as an intermediate from trans-2-nonenal treatment were detected by GC/MS spectrometer. Under acidic conditions (pH 6), lysosomal-related enzymes were the most efficient in the treatment of trans-2-nonenal. Furthermore, based on differential pH testing, we found the conditions under which all the 50 ppm trans-2-nonenal was removed. Therefore, our results suggest that the lysosomal-related enzymes reduced trans-2-nonenal, suggesting clinical application as anti-aging deodorants.

2-壬基是含有九个碳和一个不饱和键的长链脂族醛。2-清香是与衰老相关的气味的主要原因，具有令人不愉快的油腻和草味。溶酶体，线粒体和过氧化物酶体是真核细胞中重要的细胞器，其包含降解生物分子的各种水解酶。线粒体和过氧化物酶体中的蛋白质也含有醛脱氢酶。我们使用从母鸡蛋白（HEW）中提取的溶酶体相关酶进行了trans-2-nonenal治疗。由于反式-2-壬烯醛比顺式-2-壬烯醛在结构上更稳定，因此将其选为目标醛。HEW包含各种具有生物活性的蛋白质和物质，例如白蛋白，卵转铁蛋白，溶酶体，过氧化物酶体和线粒体。这里，进行了补充实验以评估溶酶体相关酶在反式2-壬烯醛治疗中的作用。溶酶体相关酶的活性已通过针对大肠杆菌。HPLC分析用于确定反式-2-壬烯醛的还原。反式-2-壬烯醛的处理取决于反应时间和酶浓度。通过GC / MS光谱仪检测被认为是反式2-壬烯醛处理中间体的物质。在酸性条件下（pH 6），溶酶体相关的酶治疗反式2-壬烯醛的效率最高。此外，基于不同的pH测试，我们发现了去除所有50 ppm反式2-壬烯醛的条件。因此，我们的结果表明，溶酶体相关的酶减少了反式-2-壬烯醛，表明其作为抗衰老除臭剂的临床应用。