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Comparative differential expression of CURS genes and determination of curcumin content at different growth stages of Curcuma longa L. and its wild relative C. zanthorrhiza Roxb.
Genetic Resources and Crop Evolution ( IF 2 ) Pub Date : 2020-06-23 , DOI: 10.1007/s10722-020-00970-z
R. Santhoshkumar , A. Yusuf

Curcuma zanthorrhiza Roxb. produces significant quantity of curcumin and essential oil in its rhizomes, hence can be used as an alternate source of curcumin. The differential expression of curcumin synthase (CURS) genes and subsequent curcumin production varied at the different developmental stages of the plant. The present study focused on the relative quantification of putative CURS (CURS1, CURS2 and CURS3) genes using qRT-PCR from the rhizomes of C. zanthorrhiza in comparison with C. longa L. and ultra fast liquid chromatography assisted curcumin quantification in both the species. The curcumin content in the rhizomes of C. zanthorrhiza were 111.6 µg/g, 352.6 µg/g, 776.2 µg/g and in C. longa were 72.5 µg/g, 140.1 µg/g and 1705.7 µg/g at the 3rd, 6th and 9th month growth stages respectively. The relative expression of putative CURS1 was 2.39 ± 0.03–6.54 ± 0.06 fold, CURS2 was 1.74 ± 0.01–3.24 ± 0.18 fold and CURS3 was 1.61 ± 0.23–3.48 ± 0.13 fold in C. zanthorrhiza and 3.58 ± 0.13–10.4 6 ± 0.11 fold for CURS1, 1.51 ± 0.06–4.92 ± 0.10 fold for CURS2 and 1.80 ± 0.03–7.01 ± 0.14 fold for CURS3 in C. longa, during the 3rd–9th month with the highest relative expression in the six-month old rhizomes of C. zanthorrhiza and C. longa. The CURS expression was positively correlated with the curcumin content in both the species during the 3rd and 6th month, however, during the 9th month the curcumin content increased and the putative CURS gene was down-regulated. The levels of expression of CURS genes are in the order with the highest expression for the CURS1, followed by CURS3 and CURS2. Thus, it can be suggested that C. zanthorrhiza can be pertinently supersede as an alternate for C. longa.



中文翻译:

姜黄及其野生近缘种C. zanthorrhiza Roxb不同生长阶段CURS基因的比较差异表达和姜黄素含量的测定。

姜黄zanthorrhiza Roxb。在其根茎中产生大量的姜黄素和精油,因此可以用作姜黄素的替代来源。姜黄素合酶(CURS)基因的差异表达和随后的姜黄素生产在植物的不同发育阶段有所不同。目前的研究集中在推定CURS(光标1,CURS2和CURS3)使用qRT-PCR从根茎基因的相对定量C. zanthorrhiza与比较C.姜黄L.和在两个物种的超快速液相色谱辅助姜黄素量化。桔梗假单胞菌的根茎中姜黄素的含量分别为111.6 µg / g,352.6 µg / g,776.2 µg / g和龙虾在生长的第3、6和9个月分别为72.5 µg / g,140.1 µg / g和1705.7 µg / g。假定的CURS1的相对表达为2.39±0.03–6.54±0.06倍,CURS2为1.74±0.01–3.24±0.18倍,CURS3为1.11±0.23–3.48±0.13倍,在山竹中和3.58±0.13–10.4 6±0.11在第3至9个月中,C在CURS1中为CURS1的1倍,CURS2为1.51±0.06–4.92±0.10倍,而CURS3为1.80±0.03–7.01±0.14倍在六个月大的C根茎中相对表达最高zanthorrhizaC.longa 在第3和第6个月,CURS的表达与该物种中姜黄素的含量呈正相关,但是在第9个月,姜黄素的含量增加并且推定的CURS基因被下调。CURS基因的表达水平按CURS1的最高表达顺序排列,其次是CURS3和CURS2。因此,可以建议将zanthorrhrrhiza C.适当地替代为Long。C. longa的替代品。

更新日期:2020-06-24
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