当前位置: X-MOL 学术J. Biomater. Appl. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Serum cytokine profile during subcutaneous implantation of the decellularized esophagus matrix in rats.
Journal of Biomaterials Applications ( IF 2.9 ) Pub Date : 2020-06-22 , DOI: 10.1177/0885328220935037
Karina Melkonyan 1 , Ramazan Nakokhov 1 , Tatyana Rusinova 1 , Yana Yutskevich 1 , Ilya Bykov 1 , Andrey Redko 1 , Sergey Alekseenko 1
Affiliation  

Aim

to study non-specific immune response characteristics (serum cytokine profile) in rats after subcutaneous implantation of the decellularized esophagus matrix.

Methods

Data were obtained in Wistar rats. The rats underwent subcutaneous implantation of decellularized esophagus (DE) and native allogeneic esophagus (NE). Explantation of sampling were carried out on the 7th, 14th and 21st day of the experiment. Explanted NEs and DEs were processed for histologic examination. The content of IL1α, IL2, IL4, IL17А, TNFα, IFNγ and GM-CSF in serum samples were tested by ELISA.

Results

In rat serum with DEs on the 7th day of the experiment it was significant increase in IL1α level in comparison with control group, IL2, TNFα, IL4 levels did not differ from the control group levels that indicates the stabilization of inflammation. The content of IL17A, IFNγ and GM-CSF significantly decreased compared to control. On the 14th day, IL17A concentration analysis showed a sharp decrease in comparison with the the 7th experimental day. We found decrease in IL1α level vs control group and decrease in IFNγ level vs 7th day. On the 21st experimental day was shown a significant decrease in the IL17A, IFNγ and IL1α content in DE rats.

Conclusions

It was found dynamic change in studied rat cytokine concentrations that correspond to favourable clinical picture in DE group in comparison with an active inflammatory reaction in NE group. IL1α, IL4, IL17A and IFNγ concentrations reflect positive dynamics of the wound healing process and the absence of local inflammation and rejection of decellularized matrices.



中文翻译:

大鼠脱细胞食管基质皮下植入过程中的血清细胞因子谱。

目的

研究皮下植入脱细胞食管基质后大鼠的非特异性免疫反应特征(血清细胞因子谱)。

方法

数据是在 Wistar 大鼠中获得的。这些大鼠经历了脱细胞食管(DE)和天然同种异体食管(NE)的皮下植入。在实验的第7天、第14天和第21天进行取样的解释。对移植的 NE 和 DE 进行组织学检查。通过ELISA检测血清样品中IL1α、IL2、IL4、IL17-、TNFα、IFNγ和GM-CSF的含量。

结果

在实验的第 7 天,在具有 DEs 的大鼠血清中,与对照组相比,IL1α 水平显着增加,IL2、TNFα、IL4 水平与对照组水平没有差异,表明炎症稳定。与对照相比,IL17A、IFNγ和GM-CSF的含量显着降低。在第14天,IL17A浓度分析显示与第7天实验相比急剧下降。我们发现 IL1α 水平与对照组相比下降,而 IFNγ 水平与第 7 天相比下降。在第 21 个实验日,DE 大鼠的 IL17A、IFNγ 和 IL1α 含量显着降低。

结论

发现与 NE 组中活跃的炎症反应相比,DE 组中研究的大鼠细胞因子浓度的动态变化与有利的临床情况相对应。IL1α、IL4、IL17A 和 IFNγ 浓度反映了伤口愈合过程的积极动态以及没有局部炎症和脱细胞基质的排斥。

更新日期:2020-06-30
down
wechat
bug