The genetic diversity of enterovirus G (EV-G) was investigated in the wild-boar population in Japan. EV-G-specific reverse transcription PCR demonstrated 30 (37.5 %) positives out of 80 faecal samples. Of these, viral protein 1 (VP1) fragments of 20 samples were classified into G1 (3 samples), G4 (1 sample), G6 (2 samples), G8 (4 samples), G11 (1 sample), G12 (7 samples), G14 (1 sample) and G17 (1 sample), among which 11 samples had a papain-like cysteine protease (PL-CP) sequence, believed to be the first discoveries in G1 (2 samples) or G17 (1 sample) wild-boar EV-Gs, and in G8 (2 samples) or G12 (6 samples) EV-Gs from any animals. Sequences of the non-structural protein regions were similar among EV-Gs possessing the PL-CP sequence (PL-CP EV-Gs) regardless of genotype or origin, suggesting the existence of a common ancestor for these strains. Interestingly, for the two G8 and two G12 samples, the genome sequences contained two versions, with or without the PL-CP sequence, together with the homologous 2C/PL-CP and PL-CP/3A junction sequences, which may explain how the recombination and deletion of the PL-CP sequences occured in the PL-CP EV-G genomes. These findings shed light on the genetic plasticity and evolution of EV-G.

中文翻译：

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在日本野猪的粪便样本中检测到的肠道病毒G的遗传多样性：鉴定带有木瓜蛋白酶样半胱氨酸蛋白酶序列的新基因型。

在日本野猪种群中调查了肠道病毒G（EV-G）的遗传多样性。EV-G特异性逆转录PCR在80份粪便样品中显示30份（37.5％）阳性。其中，将20个样品的病毒蛋白1（VP1）片段分为G1（3个样品），G4（1个样品），G6（2个样品），G8（4个样品），G11（1个样品），G12（7个样品）。 ），G14（1个样品）和G17（1个样品），其中11个样品具有木瓜蛋白酶样半胱氨酸蛋白酶（PL-CP）序列，被认为是G1（2个样品）或G17（1个样品）中的第一个发现。野猪EV-G，以及来自任何动物的G8（2个样品）或G12（6个样品）EV-G。在具有PL-CP序列的EV-G中，非结构蛋白区域的序列相似（PL-CP EV-Gs），而与基因型或起源无关，这表明这些菌株存在共同的祖先。有趣的是，对于两个G8和两个G12样品，基因组序列包含两个版本（带有或不带有PL-CP序列）以及同源的2C / PL-CP和PL-CP / 3A连接序列，这可以解释PL-CP EV-G基因组中发生了PL-CP序列的重组和缺失。这些发现揭示了EV-G的遗传可塑性和进化。