Nerve growth factor (NGF) has been proved to play important roles in male reproductive physiology, but the molecular mechanisms of NGF action remain unclear. In this study, the effects of NGF on the growth of newborn bovine testicular Sertoli (NBS) cells and the related signaling pathways were investigated. The NBS cells were treated in vitro with NGF (100 ng/ml) for 18 h. The expression levels of cell proliferation related genes, inhibin B, and cytoplasmic specialization related gene were determined using real-time PCR and western blot. The roles of PI3K/AKT and MAPK/ERK pathways in NGF-induced cell proliferation were investigated. It was found that NGF regulates proliferation and function of NBS cells via its receptor TrkA by activating the PI3K/ATK and MAPK/ERK signaling pathways. The study will help to further understand the role of NGF in male reproduction and provide new therapeutic targets for reproductive dysfunctions in male animals.

中文翻译：

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神经生长因子对新生牛睾丸支持细胞增殖的影响

神经生长因子 (NGF) 已被证明在男性生殖生理学中发挥重要作用，但 NGF 作用的分子机制尚不清楚。在本研究中，研究了 NGF 对新生牛睾丸支持 (NBS) 细胞生长的影响及相关信号通路。NBS 细胞在体外用 NGF (100 ng/ml) 处理 18 小时。使用实时PCR和蛋白质印迹测定细胞增殖相关基因、抑制素B和细胞质特化相关基因的表达水平。研究了 PI3K/AKT 和 MAPK/ERK 通路在 NGF 诱导的细胞增殖中的作用。发现NGF通过其受体TrkA通过激活PI3K/ATK和MAPK/ERK信号通路调节NBS细胞的增殖和功能。