Although development of antimicrobial resistance by bacteria is a natural phenomenon, the antibiotic resistance crisis is a reality that leads us in a gap of antimicrobial alternatives on therapeutics. Considering this cruel reality and committed to contribute to look for new antibacterial agents, this manuscript presents a review of easy laboratory methods that evaluate the mode of action of compounds, since it is a basic requirement for the discovery and development of new drugs. The literature was screened by searching the keywords “mode of action”, “antibiotic”, “antimicrobial activity”, “inhibition mode”, “method” and “bacteria” in Pubmed, Scopus, Science Direct and BVS in a period of time from 2000 to 2019. This review demonstrates the wide variety of methods that can be employed in research on mechanisms of action of antibacterial substances. Therefore, we compiled different protocols (presented in the supplementary material), available in the literature, with the purpose of facilitating the start of experiments.

中文翻译：

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寻找抗菌药物的作用机制

尽管细菌产生抗菌素耐药性是一种自然现象，但抗生素耐药性危机是一个现实，导致我们在治疗方法上存在抗菌替代品的空白。考虑到这一残酷的现实并致力于寻找新的抗菌剂，本手稿回顾了评估化合物作用模式的简单实验室方法，因为这是发现和开发新药的基本要求。通过在 Pubmed、Scopus、Science Direct 和 BVS 中搜索关键词“作用方式”、“抗生素”、“抗菌活性”、“抑制方式”、“方法”和“细菌”，从2000 年至 2019 年。本综述展示了可用于研究抗菌物质作用机制的多种方法。因此，我们编制了文献中提供的不同协议（在补充材料中提供），目的是促进实验的开始。