Magnolia officinalis bark is a traditional Chinese medicine for gastrointestinal tract disorders. In this study, we explored the effects of M. officinalis extraction on intestinal flora to reveal its mechanism. Thirty SPF mice were divided into five groups: C (control), M (M. officinalis), A (antibiotics: cefradine and gentamicin sulfate), A&M (antibiotics + M. officinalis) and A&N (antibiotics + natural recovery). Faecal samples of all groups were collected and the taxonomic composition and diversity of bacteria was characterized using the 16S rRNA gene (16S). Alpha diversity showed gut bacteria diversity significantly decreased in the A group of mice but increased markedly after administration of M. officinalis extract. Beta diversity indicated that C, M and A&M shared similar bacterial community structure while A and A&N exhibited a different bacterial community. Furthermore, RDA combined with spearman correlation heatmap suggested the five physiological indicators (weight, fur, activity and feces) were highly correlated with bacterial community structure and diversity. Finally, functional categorization of the assigned OTUs was performed using the PICRUSt tool. The changes in PICRUSt inferred that function profile and metabolic pathways were observed in A and A&M, therefore the M. officinalis extract improved the intestinal flora of A&M and normalized its metabolic pathways gradually, improving mouse weight, fur quality, activity and feces qualities.

中文翻译：

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厚朴提取物对抗生素所致小鼠肠道菌群失调的治疗作用

厚朴树皮是治疗胃肠道疾病的中药。在这项研究中，我们探索了厚朴提取物对肠道菌群的影响，以揭示其机制。30 只 SPF 小鼠被分为五组：C（对照）、M（M. officinalis）、A（抗生素：头孢拉定和硫酸庆大霉素）、A&M（抗生素 + M. officinalis）和 A&N（抗生素 + 自然恢复）。收集所有组的粪便样本，并使用 16S rRNA 基因 (16S) 表征细菌的分类组成和多样性。Alpha 多样性显示 A 组小鼠的肠道细菌多样性显着降低，但在施用厚朴提取物后显着增加。Beta多样性表明C、M和A&M具有相似的细菌群落结构，而A和A& N 表现出不同的细菌群落。此外，RDA 结合 spearman 相关热图表明五个生理指标（重量、毛皮、活性和粪便）与细菌群落结构和多样性高度相关。最后，使用 PICRUSt 工具对指定的 OTU 进行功能分类。PICRUSt 的变化推断在 A 和 A&M 中观察到功能谱和代谢途径，因此厚朴提取物改善了 A&M 的肠道菌群并逐渐使其代谢途径正常化，改善了小鼠体重、毛皮质量、活动和粪便质量。活性和粪便）与细菌群落结构和多样性高度相关。最后，使用 PICRUSt 工具对指定的 OTU 进行功能分类。PICRUSt的变化推断在A和A&M中观察到功能谱和代谢途径，因此M. officinalis提取物改善了A&M的肠道菌群并逐渐使其代谢途径正常化，改善了小鼠体重、毛皮质量、活性和粪便质量。活性和粪便）与细菌群落结构和多样性高度相关。最后，使用 PICRUSt 工具对指定的 OTU 进行功能分类。PICRUSt 的变化推断在 A 和 A&M 中观察到功能谱和代谢途径，因此厚朴提取物改善了 A&M 的肠道菌群并逐渐使其代谢途径正常化，改善了小鼠体重、毛皮质量、活动和粪便质量。