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Inhibition of berberine hydrochloride on Candida albicans biofilm formation
Biotechnology Letters ( IF 2.7 ) Pub Date : 2020-06-15 , DOI: 10.1007/s10529-020-02938-6
Xiaoxue Huang 1 , Mingyue Zheng 2, 3 , Yuling Yi 1 , Anamica Patel 4 , Zhen Song 1 , Yan Li 1
Affiliation  

This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we found that 128 and 32 μg/mL BH significantly inhibited biofilm formation (P < 0.05). BH significantly inhibited the cellular activity in early biofilms, destroyed the microscopic morphology of C.albicans and reduced the thickness of the biofilm. Both 128 and 32 μg/mL concentration solutions of BH significantly inhibited biofilm formation (P < 0.05). We found that the inhibitory effect of BH solution was positively correlated with its concentration and 128 μg/mL BH was better than 4 μg/mL fluconazole. Additionally, the results of RT-PCR indicated that 128 and 32 μg/mL BH inhibited the expression of EFG1, HWP1, ECE1, and ALS1 (P < 0.05). The efficacy of BH in inhibiting the formation of C.albicans biofilm by killing the cells in the biofilm and destroying its structure; and the mechanism may be to down-regulate the expression of EFG1, HWP1, ECE1, and ALS1 in hyphae formation, thereby, retarding the morphological transformation of C. albicans.

中文翻译:

盐酸小檗碱对白色念珠菌生物膜形成的抑制作用

本研究旨在研究盐酸小檗碱 (BH) 对白色念珠菌 (C.albicans) ATCC10231 生物膜形成的抑制作用。本文发现 BH 的浓度与其对早期生物膜细胞活性的抑制作用呈正相关,因为我们发现 128 和 32 μg/mL BH 显着抑制生物膜形成(P < 0.05)。BH显着抑制了早期生物膜中的细胞活性,破坏了白色念珠菌的微观形态并降低了生物膜的厚度。128 和 32 μg/mL 浓度的 BH 溶液均显着抑制生物膜形成(P < 0.05)。我们发现BH溶液的抑制作用与其浓度呈正相关,128 μg/mL BH优于4 μg/mL氟康唑。此外,RT-PCR结果表明,128和32 μg/mL BH抑制EFG1、HWP1、ECE1和ALS1的表达(P < 0.05)。BH通过杀死生物膜中的细胞并破坏其结构来抑制白色念珠菌生物膜形成的功效;其机制可能是下调菌丝形成中EFG1、HWP1、ECE1和ALS1的表达,从而延缓白色念珠菌的形态转变。
更新日期:2020-06-15
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