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Polyphenols from Toona sinensiss Seeds Alleviate Neuroinflammation Induced by 6-Hydroxydopamine Through Suppressing p38 MAPK Signaling Pathway in a Rat Model of Parkinson's Disease.
Neurochemical Research ( IF 4.4 ) Pub Date : 2020-06-17 , DOI: 10.1007/s11064-020-03067-2
Wenxin Zhuang 1 , Meiyun Cai 2 , Wanzhong Li 3 , Chao Chen 2 , Yanqiang Wang 4 , E Lv 2 , Wenyu Fu 2
Affiliation  

Polyphenols from Toona sinensis seeds (PTSS) have demonstrated anti-inflammatory effects in various diseases, while the anti-neuroinflammatory effects still remain to be investigated. We aimed to investigate the effects of PTSS on Parkinson’s disease and underlying mechanisms using a rat model. We employed 6-hydroxydopamine (6-OHDA) to male Sprague Dawley (SD) rats and PC12 cells to construct the in vivo and vitro models of PD and dopaminergic (DA) neuron injury, respectively. Cell viability was detected by cell counting kit-8 (CCK-8) assay and protein levels of inflammatory mediators and some p38 MAPK pathway molecules were investigated by immunohistochemistry and Western blot analyses. The results showed that 6-OHDA significantly increased protein levels of inflammatory mediators, such as cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and tumor necrosis factor α (TNF-α), which could be reversed by PTSS through suppressing the p38 MAPK pathway. The anti-inflammatory effects of PTSS were significantly enhanced by the specific p38 inhibitor of SB203580 in vitro. The present work suggests that PTSS can exert anti-inflammatory effects on PD models, which may be attributed to the suppression of p38 MAPK signaling pathway.



中文翻译:

香椿种子中的多酚通过抑制帕金森氏病大鼠模型中的p38 MAPK信号通路减轻6-羟基多巴胺诱导的神经炎症。

香椿中的多酚种子(PTSS)已在多种疾病中表现出抗炎作用,而抗神经炎作用仍有待研究。我们旨在使用大鼠模型研究PTSS对帕金森氏病的影响及其潜在机制。我们对雄性Sprague Dawley(SD)大鼠和PC12细胞采用了6-羟基多巴胺(6-OHDA),分别构建了PD和多巴胺能(DA)神经元损伤的体内和体外模型。通过细胞计数试剂盒8(CCK-8)测定来检测细胞活力,并通过免疫组织化学和蛋白质印迹分析研究炎症介质的蛋白水平和一些p38 MAPK途径分子。结果表明,6-OHDA可显着提高炎症介质的蛋白质水平,例如环氧化酶2(COX-2),诱导型一氧化氮合酶(iNOS),肿瘤坏死因子α(TNF-α),可通过抑制p38 MAPK途径被PTSS逆转。体外SB203580的特异性p38抑制剂可显着增强PTSS的抗炎作用。目前的工作表明PTSS可以对PD模型发挥抗炎作用,这可能归因于p38 MAPK信号通路的抑制。

更新日期:2020-08-12
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