A silver nanoparticle (AgNP) producing, Gram stain-positive, aerobic, motile, and rod-shaped novel bacterial strain, designated as MAH-34 T was isolated from rhizospheric soil of magnolia tree. The colonies were creamy white, smooth, circular, and 0.9–2.0 mm in diameter when grown on R2A agar. Strain MAH-34 T was found to be able to grow at 10–37 °C, at pH 6.0–9.5, and at 0–1% NaCl. The strain showed activity for both catalase, and oxidase tests, and was able to rapid synthesis of AgNPs. The TEM image revealed the spherical shape of biosynthesized AgNPs, and the size was 5 to 15 nm. Based on 16S rRNA gene sequence comparisons, the isolate was shown to be a member of genus Paenibacillus, and the close type strains were Paenibacillus chondroitinus DSM 5051 T (98.3%), Paenibacillus aceris KUDC4121T (98.2%), Paenibacillus nebraskensis JJ-59 T (97.8%), Paenibacillus alginolyticus DSM 5050 T (97.6%), Paenibacillus ferrarius CY1T (97.4%), Paenibacillus frigoriresistens YIM 016 T (97.3%), and Paenibacillus pocheonensis Gsoil 1138 T (97.3%). Strain MAH-34 T had a genome size of 8,647,010 bp. The genomic G + C content was 46.0 mol %. The major isoprenoid quinone was determined as menaquinone-7 (MK-7). The major cellular fatty acids were determined as C15:0 anteiso, and C16:0 iso. Based on the DNA-DNA hybridization results, genotypic analysis, chemotaxonomic, and physiological data, strain MAH-34 T represents a novel species, for which the name Paenibacillus anseongense sp. nov. is proposed, with MAH-34 T as the type strain (= KACC 19974 T = CGMCC1.16610 T).

中文翻译：

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Paenibacillus anseongense sp. 十一月 银纳米粒子产生菌从根际土壤中分离

银纳米粒子（AgNP）制造，革兰氏染色阳性，好氧，游动，和棒状新颖细菌菌株，命名为MAH-34 T为从木兰树的根际土壤中分离。的菌落乳白，平滑的，圆形的，和0.9-2.0毫米的直径时上R2A琼脂生长。应变MAH-34 T为发现能够在10-37℃生长，在pH 6.0-9.5，并在0-1％的NaCl。该菌株表现出活性过氧化氢酶，酶和氧化酶试验，并能的AgNPs的快速合成。TEM图像显示生物合成的AgNPs的球形形状，并且大小为5至15纳米。基于16S rRNA基因序列比较，该分离物显示出类芽孢杆菌属的一个成员，并且紧密型菌株类芽孢杆菌chondroitinus DSM 5051 T（98.3％），类芽孢杆菌aceris KUDC4121T（98.2％），类芽孢杆菌nebraskensis JJ-59Ť （97.8％），类芽孢杆菌溶藻弧菌DSM 5050 T（97.6％），类芽孢杆菌ferrarius CY1T（97.4％），类芽孢杆菌frigoriresistens YIM 016 T（97.3％），类芽孢杆菌和pocheonensis Gsoil 1138 T（97.3％）。应变MAH-34 T中8647010 bp的基因组大小。基因组G + C含量为46.0摩尔％。主要的类异戊二烯醌被确定为甲萘醌-7 (MK-7)。主要细胞脂肪酸确定为C15：0反异，和C16：0异。基于所述DNA-DNA杂交的结果，基因型分析，化学分类，和生理数据，应变MAH-34 T表示一个新种，为此，类芽孢杆菌名称anseongense藻。十一月 提出，与MAH-34 T作为型菌株（= KACC 19974 T = CGMCC1.16610 T）。和类芽孢杆菌pocheonensis Gsoil 1138 T（97.3％）。应变MAH-34 T中8647010 bp的基因组大小。基因组G + C含量为46.0摩尔％。主要的类异戊二烯醌被确定为甲基萘醌-7（MK-7）。主要细胞脂肪酸确定为C15：0反异，和C16：0异。基于所述DNA-DNA杂交的结果，基因型分析，化学分类，和生理数据，应变MAH-34 T表示一个新种，为此，类芽孢杆菌名称anseongense藻。十一月 提出，与MAH-34 T作为型菌株（= KACC 19974 T = CGMCC1.16610 T）。和类芽孢杆菌pocheonensis Gsoil 1138 T（97.3％）。应变MAH-34 T中8647010 bp的基因组大小。基因组G + C含量为46.0摩尔％。主要的类异戊二烯醌被确定为甲基萘醌-7（MK-7）。主要细胞脂肪酸确定为C15：0反异，和C16：0异。基于 DNA-DNA 杂交结果、基因型分析、化学分类学和生理学数据，菌株 MAH-34 T 代表了一个新物种，其名称为 Paenibacillus anseongense sp。十一月 提出，与MAH-34 T作为型菌株（= KACC 19974 T = CGMCC1.16610 T）。0 等。基于所述DNA-DNA杂交的结果，基因型分析，化学分类，和生理数据，应变MAH-34 T表示一个新种，为此，类芽孢杆菌名称anseongense藻。十一月 提出，与MAH-34 T作为型菌株（= KACC 19974 T = CGMCC1.16610 T）。0异。基于 DNA-DNA 杂交结果、基因型分析、化学分类学和生理学数据，菌株 MAH-34 T 代表了一个新物种，其名称为 Paenibacillus anseongense sp。十一月 提出，与MAH-34 T作为型菌株（= KACC 19974 T = CGMCC1.16610 T）。