Botrytis cinerea is a foliar necrotrophic fungal-pathogen capable of infecting >580 genera of plants, is often used as model organism for studying fungal-host interactions. We used RNAseq to study transcriptome of B. cinerea infection on a major (worldwide) vegetable crop, tomato (Solanum lycopersicum). Most previous works explored only few infection stages, using RNA extracted from entire leaf-organ diluting the expression of studied infected region. Many studied B. cinerea infection, on detached organs assuming that similar defense/physiological reactions occurs in the intact plant. We analyzed transcriptome of the pathogen and host in 5 infection stages of whole-plant leaves at the infection site. We supply high quality, pathogen-enriched gene count that facilitates future research of the molecular processes regulating the infection process.

灰葡萄孢（Botrytis cinerea）是一种叶片坏死性真菌病原体，能够感染> 580属植物，通常用作研究真菌与宿主相互作用的模型生物。我们使用RNA测序来研究转录灰霉病菌感染上的一大（全球）蔬菜作物，番茄（番茄）。以前的大多数工作都使用从整个叶片器官中提取的RNA稀释了所研究感染区域的表达来探索仅几个感染阶段。许多研究灰葡萄孢假定完整的植物中发生了类似的防御/生理反应，则在分离的器官上感染。我们在感染部位的全株叶片的5个感染阶段分析了病原体和宿主的转录组。我们提供高质量的，病原体丰富的基因计数，这有助于将来对调节感染过程的分子过程进行研究。