BACKGROUND The accuracy of glucose meters is evaluated by comparing their results with those from a reference laboratory glucose analyser. The main scientific societies recommend the use of a prompt glycolysis inhibitor such as citrate for an accurate glucose determination. In the present preliminary study, we discuss the bias between capillary and plasma glucose measured concentrations, determined in two Italian clinical laboratories, using tubes containing an NaF and citrate mixture in liquid and granular form. METHODS 139 volunteers in whom 75 g OGTT was requested were recruited. Basal capillary glucose was determined using Abbott FreeStyle Precision Neo in Brescia (n=63), while clinical laboratory reference P-glucose was determined using tubes containing NaF/K3EDTA and liquid NaF/Na2EDTA/citrate. Basal capillary glucose was determined using a Roche Cobas Accu-Chek Inform II in Vicenza (n=76), while P-glucose was determined using tubes containing NaF/K2Ox and NaF/Na2EDTA/citrate in granulated form. Reference P-glucose was determined with a hexokinase method on Dimension Vista systems. Differences between capillary and reference P-glucose were evaluated according to ADA/ISO 15197:2013 specifications. RESULTS 96.82% and 97.37% of capillary determinations were within specifications when liquid and granular citrate mixture tubes were used, respectively. Conversely, only 73.02% and 80.26% of determinations were within criteria using NaF. CONCLUSIONS It's important to know what is the laboratory reference glucose in evaluating glucose meters' accuracy. The evaluation of glucometers' accuracy with respect to a reference laboratory may be wrong if tubes containing only NaF are used due to in vitro glycolysis. Only tubes containing citrate mixture permit the correct evaluation of glucose meters' accuracy.

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血糖仪：什么是实验室参考葡萄糖？

背景技术葡萄糖计的准确度是通过将它们的结果与参考实验室葡萄糖分析仪的结果进行比较来评估的。主要科学协会推荐使用快速糖酵解抑制剂（如柠檬酸盐）来准确测定葡萄糖。在目前的初步研究中，我们讨论了毛细血管和血浆葡萄糖测量浓度之间的偏差，这些浓度是在两个意大利临床实验室使用含有液体和颗粒形式的 NaF 和柠檬酸盐混合物的试管确定的。方法 招募了 139 名要求 75 克 OGTT 的志愿者。在布雷西亚 (n=63) 使用 Abbott FreeStyle Precision Neo 测定基础毛细血管葡萄糖，而使用含有 NaF/K3EDTA 和液体 NaF/Na2EDTA/柠檬酸盐的试管测定临床实验室参考 P-葡萄糖。在 Vicenza (n=76) 使用 Roche Cobas Accu-Chek Inform II 测定基础毛细血管葡萄糖，而使用含有颗粒形式的 NaF/K2Ox 和 NaF/Na2EDTA/柠檬酸盐的试管测定 P-葡萄糖。在 Dimension Vista 系统上使用己糖激酶法测定参比 P-葡萄糖。根据 ADA/ISO 15197:2013 规范评估毛细管和参考 P-葡萄糖之间的差异。结果 当使用液体和颗粒柠檬酸盐混合物试管时，分别有 96.82% 和 97.37% 的毛细管测定符合规格。相反，只有 73.02% 和 80.26% 的测定符合使用 NaF 的标准。结论 了解什么是实验室参考葡萄糖对评估血糖仪的准确性很重要。血糖仪的评价 如果由于体外糖酵解而使用仅包含 NaF 的试管，参考实验室的准确性可能是错误的。只有含有柠檬酸盐混合物的试管才能正确评估血糖仪的准确性。