Glioblastoma multiform (GBM) continues to threaten people's lives due to the limited therapeutic strategies. As a new drug, Valerenic Acid suppresses the progression of GBM, however, the mechanism is largely unknown. Here, we found that Valerenic Acid can inhibit cell proliferation, migration and invasion of GBM cells by increasing innate immune signals such as enhancing ROS levels and activating the AMPK pathway. Inhibition of ROS by N-acetylcysteine (NAC) or attenuation of AMPK by Compound C could block Valerenic Acid-induced cell death. Additionally, the xenograft mouse model also confirmed that Valerenic Acid had anti-tumor effect. Together, our results provide compelling rational to develop Valerenic Acid as an anti-tumor agent against GBM patients.

中文翻译：

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5-HT 受体激动剂 Valerenic Acid 可增强先天免疫信号并抑制胶质母细胞瘤细胞的生长和侵袭。

由于治疗策略有限，多形性胶质母细胞瘤 (GBM) 继续威胁着人们的生命。作为一种新药，缬草酸能够抑制 GBM 的进展，但其机制尚不清楚。在这里，我们发现缬草酸可以通过增加先天免疫信号如增强 ROS 水平和激活 AMPK 通路来抑制 GBM 细胞的细胞增殖、迁移和侵袭。N-乙酰半胱氨酸 (NAC) 对 ROS 的抑制或化合物 C 对 AMPK 的减弱可以阻止缬草酸诱导的细胞死亡。此外，异种移植小鼠模型也证实了缬草酸具有抗肿瘤作用。总之，我们的结果为开发缬草酸作为针对 GBM 患者的抗肿瘤剂提供了令人信服的理由。