Pooled genetic screening is a powerful method to systematically link genotype to phenotype and gain insights into biological processes, but applying it to visual phenotypes such as cell morphology or protein localization has remained a challenge. In their recent work, Fowler and colleagues (Hasle et al , 2020) describe an elegant approach for high‐throughput cell sorting according to visual phenotypes based on selective photoconversion. This allows combining the advantages of high‐content phenotyping by fluorescence microscopy with the efficiency of pooled screening to dissect complex phenotypes.

中文翻译：

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（照片）转换为汇总视觉筛选。

汇集遗传筛查是一种强大的方法，可以系统地将基因型与表型联系起来并深入了解生物过程，但将其应用于细胞形态或蛋白质定位等视觉表型仍然是一个挑战。在他们最近的工作中，Fowler 和同事（Hasle等人，2020）描述了一种根据基于选择性光转换的视觉表型进行高通量细胞分选的优雅方法。这使得可以将荧光显微镜高内涵表型分析的优点与汇总筛选的效率结合起来，以剖析复杂的表型。