Profound physiological changes during pregnancy may affect the requirement of retinol and tocopherol, which are essential micronutrients for the maintenance of maternal health and foetal development. However, the current reference intervals (RIs) of retinol and tocopherol are based on non-pregnant population. In the present study, a liquid chromatography-tandem mass spectrometry quantitation method for serum retinol and α-tocopherol was established and validated. In addition, we established trimester-specific RIs of retinol and α-tocopherol using the data from paired screening test for 31,301 outpatients who participated in the prenatal vitamins A/E evaluation program at our hospital using the Hoffmann method, which is a simple indirect RI estimation method that does not require the recruitment of healthy subjects. Further, to explore the associations between the levels of retinol and α-tocopherol and the parameters of complete blood count (CBC), the results of retinol, α-tocopherol, and CBC of 1,977 pregnant outpatients in the third trimester were analysed. The testing interval between the levels of vitamins and CBC was no more than 7 days. Although no significant changes were noticed in the levels of retinol, the α-tocopherol levels continuously increased with normal physiological changes throughout pregnancy. Lower retinol levels were associated with the higher incidence of anaemia, whereas higher levels of retinol and lower levels of α-tocopherol were associated with higher platelet count.

中文翻译：

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妊娠中的视黄醇和α-生育酚：参考区间的建立和与CBC的关联。

怀孕期间发生深刻的生理变化可能会影响视黄醇和生育酚的需求，这是维持母体健康和胎儿发育必不可少的微量营养素。但是，视黄醇和生育酚的当前参考间隔（RIs）是基于非妊娠人群。本研究建立并验证了血清视黄醇和α-生育酚的液相色谱-串联质谱定量方法。此外，我们使用Hoffmann方法（通过简单的间接RI）使用31 301名参与我院产前维生素A / E评估程序的门诊患者的配对筛查数据，建立了视黄醇和α-生育酚的孕中期特异性RI。不需要招募健康受试者的估算方法。进一步，为了探讨视黄醇和α-生育酚水平与全血细胞计数（CBC）参数之间的关系，分析了妊娠中期三个月的1,977名孕妇门诊的视黄醇，α-生育酚和CBC结果。维生素和CBC水平之间的测试间隔不超过7天。尽管未观察到视黄醇水平的显着变化，但在整个妊娠期间，α-生育酚水平会随着正常的生理变化而持续增加。较低的视黄醇水平与较高的贫血发生率相关，而较高的视黄醇水平和较低的α-生育酚水平与较高的血小板计数相关。分析了妊娠中期的977名孕妇门诊。维生素和CBC水平之间的测试间隔不超过7天。尽管未观察到视黄醇水平的显着变化，但在整个怀孕期间，α-生育酚水平会随着正常的生理变化而持续增加。较低的视黄醇水平与较高的贫血发生率相关，而较高的视黄醇水平和较低的α-生育酚水平与较高的血小板计数相关。分析了妊娠中期的977名孕妇门诊。维生素和CBC水平之间的测试间隔不超过7天。尽管未观察到视黄醇水平的显着变化，但在整个怀孕期间，α-生育酚水平会随着正常的生理变化而持续增加。较低的视黄醇水平与较高的贫血发生率相关，而较高的视黄醇水平和较低的α-生育酚水平与较高的血小板计数相关。