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Molecular insights into the powerful mucus-based adhesion of limpets (Patella vulgata L.).
Open Biology ( IF 5.8 ) Pub Date : 2020-06-17 , DOI: 10.1098/rsob.200019 Victor Kang 1 , Birgit Lengerer 2, 3 , Ruddy Wattiez 4 , Patrick Flammang 2
Open Biology ( IF 5.8 ) Pub Date : 2020-06-17 , DOI: 10.1098/rsob.200019 Victor Kang 1 , Birgit Lengerer 2, 3 , Ruddy Wattiez 4 , Patrick Flammang 2
Affiliation
Limpets (Patella vulgata L.) are renowned for their powerful attachments to rocks on wave-swept seashores. Unlike adult barnacles and mussels, limpets do not adhere permanently; instead, they repeatedly transition between long-term adhesion and locomotive adhesion depending on the tide. Recent studies on the adhesive secretions (bio-adhesives) of marine invertebrates have expanded our knowledge on the composition and function of temporary and permanent bio-adhesives. In comparison, our understanding of the limpets' transitory adhesion remains limited. In this study, we demonstrate that suction is not the primary attachment mechanism in P. vulgata; rather, they secrete specialized pedal mucus for glue-like adhesion. Through combined transcriptomics and proteomics, we identified 171 protein sequences from the pedal mucus. Several of these proteins contain conserved domains found in temporary bio-adhesives from sea stars, sea urchins, marine flatworms and sea anemones. Many of these proteins share homology with fibrous gel-forming glycoproteins, including fibrillin, hemolectin and SCO-spondin. Moreover, proteins with potential protein- and glycan-degrading domains could have an immune defence role or assist degrading adhesive mucus to facilitate the transition from stationary to locomotive states. We also discovered glycosylation patterns unique to the pedal mucus, indicating that specific sugars may be involved in transitory adhesion. Our findings elucidate the mechanisms underlying P. vulgata adhesion and provide opportunities for future studies on bio-adhesives that form strong attachments and resist degradation until necessary for locomotion.
中文翻译:
分子洞察力表明强大的基于粘液的帽贝(P)。
残肢(Patella vulgata L.)因其对波涛汹涌的海边岩石的强大附着力而闻名。与成年的藤壶和贻贝不同,帽贝不会永久附着。相反,它们会根据潮汐在长期附着力和机车附着力之间反复转换。关于海洋无脊椎动物的粘合剂分泌物(生物粘合剂)的最新研究扩展了我们对临时和永久性生物粘合剂的组成和功能的了解。相比之下,我们对帽毛的短暂粘附的理解仍然有限。在这项研究中,我们证明吸力不是寻常的主要附着机制。相反,他们分泌专门的踏板粘液以产生类似胶的粘着力。通过结合转录组学和蛋白质组学,我们从踏板粘液中鉴定了171个蛋白质序列。这些蛋白中的几种包含在海星,海胆,海洋扁虫和海葵的临时生物粘合剂中发现的保守结构域。这些蛋白中的许多与形成纤维的凝胶糖蛋白具有同源性,包括原纤维蛋白,血凝素和SCO-spondin。此外,具有潜在的蛋白质和聚糖降解结构域的蛋白质可能具有免疫防御作用,或有助于降解粘液,以促进从固定状态向机车状态的转变。我们还发现踏板黏液特有的糖基化模式,表明特定的糖可能参与短暂的粘连。我们的发现阐明了体育假单胞菌黏附的机制,并为生物黏附剂的未来研究提供了机会,这些生物黏附剂形成牢固的附着物并抵抗降解直至运动所需。
更新日期:2020-06-17
中文翻译:
分子洞察力表明强大的基于粘液的帽贝(P)。
残肢(Patella vulgata L.)因其对波涛汹涌的海边岩石的强大附着力而闻名。与成年的藤壶和贻贝不同,帽贝不会永久附着。相反,它们会根据潮汐在长期附着力和机车附着力之间反复转换。关于海洋无脊椎动物的粘合剂分泌物(生物粘合剂)的最新研究扩展了我们对临时和永久性生物粘合剂的组成和功能的了解。相比之下,我们对帽毛的短暂粘附的理解仍然有限。在这项研究中,我们证明吸力不是寻常的主要附着机制。相反,他们分泌专门的踏板粘液以产生类似胶的粘着力。通过结合转录组学和蛋白质组学,我们从踏板粘液中鉴定了171个蛋白质序列。这些蛋白中的几种包含在海星,海胆,海洋扁虫和海葵的临时生物粘合剂中发现的保守结构域。这些蛋白中的许多与形成纤维的凝胶糖蛋白具有同源性,包括原纤维蛋白,血凝素和SCO-spondin。此外,具有潜在的蛋白质和聚糖降解结构域的蛋白质可能具有免疫防御作用,或有助于降解粘液,以促进从固定状态向机车状态的转变。我们还发现踏板黏液特有的糖基化模式,表明特定的糖可能参与短暂的粘连。我们的发现阐明了体育假单胞菌黏附的机制,并为生物黏附剂的未来研究提供了机会,这些生物黏附剂形成牢固的附着物并抵抗降解直至运动所需。