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The molecular cytogenetic characterization of Conopophaga lineata indicates a common chromosome rearrangement in the Parvorder Furnariida (Aves, Passeriformes)
Genetics and Molecular Biology ( IF 2.1 ) Pub Date : 2020-01-01 , DOI: 10.1590/1678-4685-gmb-2020-0018
Thays Duarte de Oliveira 1 , Rafael Kretschmer 2 , Natasha Ávila Bertocchi 2 , Patricia C M O'Brien 3 , Malcolm A Ferguson-Smith 3 , Analía Del Valle Garnero 4 , Edivaldo Herculano Correa de Oliveira 5, 6 , Ricardo José Gunski 4
Affiliation  

Abstract Cytogenetic analyses of the Suboscines species are still scarce, and so far, there is no karyotype description of any species belonging to the family Conopophagidae. Thus, the aim of this study is to describe and analyze the karyotype of Conopophaga lineata by chromosome painting using Gallus gallus (GGA) probes and to identify the location of the 18/28S rDNA cluster. Metaphases were obtained from fibroblast culture from two individuals of C. lineata. We observed a diploid number of 2n=78. GGA probes showed that most ancestral syntenies are conserved, except for the fission of GGA1 and GGA2, into two distinct pairs each. We identified the location of 18S rDNA genes in a pair of microchromosomes. The fission of the syntenic group corresponding to GGA2 was observed in other Furnariida, and hence may correspond to a chromosomal synapomorphy for the species of Parvorder Furnariida.

中文翻译:

Conopophaga lineata 的分子细胞遗传学特征表明 Parvorder Furnariida (Aves, Passeriformes) 中常见的染色体重排

摘要 Suboscines 物种的细胞遗传学分析仍然很少,到目前为止,还没有属于 Conopophagidae 科的任何物种的核型描述。因此,本研究的目的是通过使用鸡 (GGA) 探针进行染色体染色来描述和分析 Conopophaga lineata 的核型,并确定 18/28S rDNA 簇的位置。从 C. lineata 的两个个体的成纤维细胞培养物中获得中期。我们观察到二倍体数为 2n=78。GGA 探针表明,除了 GGA1 和 GGA2 的裂变外,大多数祖先的同线性都是保守的,每个都分为两个不同的对。我们确定了一对微染色体中 18S rDNA 基因的位置。在其他 Furnariida 中观察到与 GGA2 对应的同线基团的裂变,
更新日期:2020-01-01
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