Let alone calorie restriction, life span extension in higher organisms has proven to be difficult to achieve using simple drugs. Previous studies have shown that the polyamine spermidine increased the maximum life span in C. elegans and the median life span in mice. However, younger subjects (< 40 years of age) are infrequently prescribed nor self-medicating with antiaging drugs. Therefore, in the present study, we aimed at assessing the effect of long-term treatment with spermidine given in the drinking water on behavioral performance and longevity of male, middle-aged Sprague-Dawley rats. We report that spermidine given in the drinking water did not extend neither the median nor the maximum life span of the middle-aged male Sprague-Dawley rats. However, spermidine treatment had a beneficial effect on the body weight and the kidney tubules, liver, and heart morphology. Behaviorally, spermidine led to a reduction in anxiety and an increase in curiosity, as assessed by exploratory behavior. Moreover, long-term treatment with spermidine enhanced autophagy in the brain and led to a diminished expression of the inflammatory markers, Tgfb, CD11b, Fcgr1, Stat1, CR3, and GFAP mRNAs in several cortical region and hippocampus of the treated rats suggesting that one beneficial effect of the long-term treatment with spermidine is an attenuated proinflammatory state in the aged brain. Our results suggest that long-term treatment with spermidine increases health span of middle-aged rats by attenuating neuroinflammation and improving anxiety and exploratory behavior.

中文翻译：

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亚精胺的长期治疗可延长中年Sprague-Dawley雄性大鼠的健康寿命。

更不用说限制卡路里了，使用简单的药物很难达到延长高等生物的寿命的目的。先前的研究表明，多胺亚精胺可增加秀丽隐杆线虫的最大寿命。和小鼠的平均寿命。但是，不经常给年轻的受试者（<40岁）开处方，也不能自行服用抗衰老药物。因此，在本研究中，我们旨在评估饮用水中长期给予亚精胺对雄性，中年Sprague-Dawley大鼠行为表现和寿命的影响。我们报告在饮用水中给予亚精胺既不会延长中年雄性Sprague-Dawley大鼠的中位寿命，也不会延长其最大寿命。然而，亚精胺治疗对体重和肾小管，肝脏和心脏形态具有有益的作用。从行为上看，通过探索性行为评估，亚精胺可导致焦虑减轻和好奇心增加。此外，Tgfb，CD11b，Fcgr1，Stat1，CR3和GFAP mRNA在被治疗大鼠的几个皮质区域和海马中显示，这表明长期用亚精胺治疗的有益作用是减轻衰老的大脑的促炎状态。我们的结果表明，长期使用亚精胺治疗可通过减轻神经炎症，改善焦虑和探索行为来延长中年大鼠的健康期。