Fabrication of osteoconductive scaffold with osteoinductive capability and appropriate resorption rate is of great significance for treating bone defects. To achieve this aim, strontium-substituted calcium sulfate hemihydrate (Sr-CSH) and hydroxyapatite (HA) were mixed to develop a novel composite. Sr-CSH containing 5% and 10% strontium was mixed with HA at the weight ratio of 6:4, respectively. Female Sprague-Dawley rats underwent bone defect surgery in left tibia were randomly assigned to three different treatment groups filled with CSH/HA, 5% and 10% Sr-CSH/HA. Micro-CT analysis showed increased new bone formation in 10% Sr-CSH/HA group compared to CSH/HA group. In addition, histological analysis showed large amounts of chondrocytes and osteoblasts within the pores of Sr-CSH/HA composites as a result of the CSH resorption. Further, CFU-F assay demonstrated the increased amount of bone marrow mesenchymal stromal cells (BMSCs) colonies in 10% Sr-CSH/HA group. In primary BMSCs, extraction from Sr-CSH/HA composite significantly increased the migration of cells, up-regulated the expression of osteoblastic marker genes, and increased the area of mineralized nodules. Together, Sr-CSH/HA may promote bone formation by recruiting and stimulating osteogenic differentiation of BMSCs. Therefore, this composite may be proposed as an ideal substitute to repair bone defects.

制备具有骨诱导能力和适当骨吸收率的骨传导支架对治疗骨缺损具有重要意义。为了实现这一目标，将锶取代的半水合硫酸钙（Sr-CSH）和羟基磷灰石（HA）混合以开发一种新型复合材料。分别以 6:4 的重量比将含有 5% 和 10% 锶的 Sr-CSH 与 HA 混合。将接受左胫骨骨缺损手术的雌性 Sprague-Dawley 大鼠随机分配到三个不同的治疗组，分别填充 CSH/HA、5% 和 10% Sr-CSH/HA。Micro-CT 分析显示，与 CSH/HA 组相比，10% Sr-CSH/HA 组的新骨形成增加。此外，组织学分析表明，由于 CSH 吸收，在 Sr-CSH/HA 复合材料的孔中存在大量软骨细胞和成骨细胞。更远，CFU-F 测定表明 10% Sr-CSH/HA 组中骨髓间充质基质细胞 (BMSCs) 集落的数量增加。在原代 BMSC 中，从 Sr-CSH/HA 复合物中提取显着增加了细胞的迁移，上调了成骨细胞标记基因的表达，并增加了矿化结节的面积。总之，Sr-CSH/HA 可以通过募集和刺激 BMSCs 的成骨分化来促进骨形成。因此，这种复合材料可作为修复骨缺损的理想替代品。总之，Sr-CSH/HA 可以通过募集和刺激 BMSCs 的成骨分化来促进骨形成。因此，这种复合材料可作为修复骨缺损的理想替代品。总之，Sr-CSH/HA 可以通过募集和刺激 BMSCs 的成骨分化来促进骨形成。因此，这种复合材料可作为修复骨缺损的理想替代品。