In this study, a transferrin/folic acid double-targeting graphene oxide drug delivery system loaded with doxorubicin was designed. Graphene oxide was prepared by ultrasound improved Hummers method and was modified with Pluronic F68, folic acid, and transferrin to decrease its toxicity and to allow dual-targeting. The results show that the double target drug delivery system (TFGP*DOX) has good and controllable drug delivery performance with no toxicity. Moreover, TFGP*DOX has a better inhibitory effect on SMMC-7721 cells than does a single target drug delivery system (FGP*DOX). The results of drug release analysis and cell inhibition studies showed that TFGP*DOX has a good sustained release function that can reduce the drug release rate in blood circulation over time and improve the local drug concentration in or near a targeted tumor. Therefore, the drug loading system (TFGP*DOX) has potential application value in the treatment of hepatocellular carcinoma.

中文翻译：

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转铁蛋白/叶酸双靶向氧化石墨烯给药系统的制备及抗癌活性研究

本研究设计了一种载有阿霉素的转铁蛋白/叶酸双靶向氧化石墨烯给药系统。氧化石墨烯是通过超声改进的 Hummers 方法制备的，并用 Pluronic F68、叶酸和转铁蛋白进行修饰以降低其毒性并允许双重靶向。结果表明，双靶点给药系统（TFGP*DOX）具有良好且可控的给药性能，且无毒性。此外，TFGP*DOX 对 SMMC-7721 细胞的抑制作用比单靶点给药系统（FGP*DOX）更好。药物释放分析和细胞抑制研究结果表明，TFGP*DOX具有良好的缓释功能，可以随着时间的推移降低血液循环中的药物释放速率，提高靶向肿瘤内或附近的局部药物浓度。所以，