Oxygen is a vital molecule for cell and tissue processes. Electrospun fibers have been extensively used as drug loading carriers due to possibility of well control over drug release with modulating fiber properties. However, they have not been used as depots for oxygen release. In the present study, an oxygen-releasing nanofibrous scaffold has been developed by electrospinning of polylactic acid/nano-calcium peroxide suspension with different polylactic acid concentrations (6.5 and 13% w/v). The electrospun fibers with calcium peroxide cargo provided oxygen content of 30-94 mmHg in a period of 14 days which lies well within the oxygen level of osseous tissue. The release profile of 13% polylactic acid fibers was different with that of 6.5% fibers with respects to the initial content of released oxygen and the release rate. Not only did 13% fibers supply oxygen with a slower rate, but also they resulted in a lower burst release of oxygen. Cell culture studies in hypoxia corroborated that 13% polylactic acid fibers better preserve cell viability comparing 6.5% counterparts as perceived by MTT assay. Moreover, they endowed more favored milieu for adherence, arrangement and migration of mesenchymal stem cells as confirmed by microscopy images. The oxygen-releasing fibers equally affected alkaline phosphatase, osteocalcin, and calcium deposition by mesenchymal stem cells most likely due to interplay between topographical and metabolic cues offered by 6.5 and 13% formulations.

中文翻译：

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用于透气性骨组织工程应用的释氧纳米纤维。

氧气是细胞和组织过程的重要分子。由于可以通过调节纤维特性很好地控制药物释放，电纺纤维已被广泛用作载药载体。然而，它们还没有被用作释放氧气的仓库。在本研究中，通过静电纺丝具有不同聚乳酸浓度（6.5 和 13% w/v）的聚乳酸/纳米过氧化钙悬浮液开发了一种释氧纳米纤维支架。带有过氧化钙货物的电纺纤维在 14 天内提供 30-94 mmHg 的氧含量，这完全在骨组织的氧含量范围内。就释放氧的初始含量和释放速率而言，13% 聚乳酸纤维的释放曲线与 6.5% 纤维的释放曲线不同。13% 的纤维不仅以较慢的速度供应氧气，而且它们导致氧气的爆发式释放较低。缺氧条件下的细胞培养研究证实，13% 的聚乳酸纤维更好地保持了细胞活力，而 MTT 检测结果显示为 6.5%。此外，它们为间充质干细胞的粘附、排列和迁移提供了更有利的环境，正如显微镜图像所证实的那样。释氧纤维同样影响碱性磷酸酶、骨钙素和间充质干细胞的钙沉积，这很可能是由于 6.5% 和 13% 配方提供的地形和代谢线索之间的相互作用。缺氧条件下的细胞培养研究证实，13% 的聚乳酸纤维更好地保持了细胞活力，而 MTT 检测结果显示为 6.5%。此外，它们为间充质干细胞的粘附、排列和迁移提供了更有利的环境，正如显微镜图像所证实的那样。释氧纤维同样影响碱性磷酸酶、骨钙素和间充质干细胞的钙沉积，这很可能是由于 6.5% 和 13% 配方提供的地形和代谢线索之间的相互作用。缺氧条件下的细胞培养研究证实，13% 的聚乳酸纤维更好地保持了细胞活力，而 MTT 检测结果显示为 6.5%。此外，它们为间充质干细胞的粘附、排列和迁移提供了更有利的环境，正如显微镜图像所证实的那样。释氧纤维同样影响碱性磷酸酶、骨钙素和间充质干细胞的钙沉积，这很可能是由于 6.5% 和 13% 配方提供的地形和代谢线索之间的相互作用。