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Two new Cu(II)-based coordination polymers: inhibitory activity on prostate cancer by reducing EGF-R expression and HIPPO signaling pathway activation.
Journal of Biomaterials Science, Polymer Edition ( IF 3.6 ) Pub Date : 2020-06-12 , DOI: 10.1080/09205063.2020.1775057
Xiang-Ying Wang 1 , Jian Xiao 2 , Hui-Qing Ma 2
Affiliation  

Under solvothermal conditions, two new coordination polymers (CPs), namely {[Cu(HL)]·2CH3CN}n (1) and {[Cu(HL)(bimb)]·4H2O}n (2) (H3L = tris(p-carboxyphenyl)phosphane oxide, bimb = 1,4-bis(imidazol-1-ylmethyl)benzene) were prepared. Their treatment activity on the human prostate cancer was evaluated through series of biological experiments. Firstly, the human prostate cancer cells proliferation was determined with Cell Counting Kit-8 (CCK-8) detection kit. The influence of compounds 1 and 2 on the cancer cells migration and invasion ability was measured with trans-well assay. Next, the important role of the EGF-R human prostate cancer cells was determined with western blotting. In the end, the activation of the HIPPO signaling pathway was detected with real-time reverse transcription polymerase chain reaction (RT-PCR).



中文翻译:

两种新的基于 Cu(II) 的配位聚合物:通过减少 EGF-R 表达和 HIPPO 信号通路激活对前列腺癌的抑制活性。

在溶剂热条件下,两种新的配位聚合物(CPs),即{[Cu(HL)]·2CH 3 CN} n ( 1 ) 和{[Cu(HL)(bimb)]·4H 2 O} n ( 2 ) (制备H 3 L = 三(对羧基苯基)氧化膦,bimb = 1,4-双(咪唑-1-基甲基)苯)。通过一系列生物学实验评估了它们对人类前列腺癌的治疗活性。首先,用Cell Counting Kit-8(CCK-8)检测试剂盒测定人前列腺癌细胞增殖情况。化合物12的影响Trans-well 法测定对癌细胞迁移和侵袭能力的影响。接下来,通过蛋白质印迹确定了 EGF-R 人前列腺癌细胞的重要作用。最后,通过实时逆转录聚合酶链反应 (RT-PCR) 检测 HIPPO 信号通路的激活。

更新日期:2020-06-12
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