Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) is a rapid, cost-effective and high-throughput method for bacteria characterization. However, most previous studies focused on clinical isolates. In this study, we evaluated the use of MALDI-TOF MS as a rapid screening tool for marine bacterial symbionts. A set of 255 isolates from different marine sources (corals, sponge, fish and seawater) was analyzed using cell lysates to obtain a rapid grouping. Cluster analysis of mass spectra and 16S rRNA showed 18 groups, including Vibrio, Bacillus, Pseudovibrio, Alteromonas and Ruegeria . MALDI-TOF distance similarity scores ≥ 60% and ≥ 70% correspond to ≥ 98.7% 16S rRNA gene sequence similarity and ≥ 95% pyrH gene sequence similarity, respectively. MALDI-TOF MS is a useful tool for Vibrio species groups’ identification.

中文翻译：

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使用 MALDI-TOF MS 快速筛选海洋细菌共生体

基质辅助激光解吸电离飞行时间质谱 (MALDI-TOF MS) 是一种快速、经济高效且高通量的细菌表征方法。然而，以前的大多数研究都集中在临床分离株上。在这项研究中，我们评估了 MALDI-TOF MS 作为海洋细菌共生体快速筛选工具的使用。使用细胞裂解物分析了一组来自不同海洋来源（珊瑚、海绵、鱼和海水）的 255 个分离物，以获得快速分组。质谱和16S rRNA的聚类分析显示18个组，包括弧菌、芽孢杆菌、假单胞菌、交替单胞菌和Ruegeria。MALDI-TOF 距离相似性得分≥ 60% 和≥ 70% 分别对应≥ 98.7% 16S rRNA 基因序列相似性和≥ 95% pyrH 基因序列相似性。