Although bisphenol A (BPA) has been associated with impaired spermatogenesis, the mechanisms remain unclear. Tight junction occludin plays important roles in spermatogenesis. The objective of the present study was to explore the effects of BPA exposure in adolescent mice. Male mice were orally treated with low-dose (0.05 mg/kg/d), middle-dose (5.0 mg/kg/d), or high-dose (50 mg/kg/d) BPA in corn oil from postnatal day (PND) 35 to 65. Animals were killed on PND 65 and PND 125. On PND 65, the sperm count, sperm motility, and the expression of occludin showed a dose-related decline. On PND 125, the sperm count, sperm motility, and the expression of occludin were in recovery. However, there remained significant decreases in these parameters in the 50 mg/kg/d group on PND 125 compared with the control. The dose-related effects on the measured parameters and occludin expression suggest an early suppressive or damaging effect on the blood–testis barrier followed by recovery after dosing ceased. At a BPA dose of 50 mg/kg/d, recovery did not occur, suggesting that higher doses of BPA may cause irreversible damage to reproduction in male mice.

尽管双酚A（BPA）与精子发生受损有关，但机制尚不清楚。紧密连接的occludin在精子发生中起重要作用。本研究的目的是探讨BPA暴露对青春期小鼠的影响。从出生后开始，对雄性小鼠口服玉米油中的低剂量（0.05 mg / kg / d），中剂量（5.0 mg / kg / d）或高剂量（50 mg / kg / d）BPA（ （PND）35到65。在PND 65和PND 125处杀死动物。在PND 65上，精子数量，精子运动性和闭合蛋白的表达呈剂量相关性下降。在PND 125上，精子数量，精子活力和闭合蛋白的表达恢复。但是，与对照组相比，PND 125上50 mg / kg / d组的这些参数仍然明显降低。剂量相关参数对测量参数和闭合蛋白表达的影响表明对血液-睾丸屏障的早期抑制或破坏作用，然后在停药后恢复。在BPA剂量为50 mg / kg / d时，未出现恢复，这表明较高剂量的BPA可能对雄性小鼠的繁殖造成不可逆的损害。