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Probiotic and technological features of Enterococcus and Weissella isolates from camel milk characterised by an Argane feeding regimen
Archives of Microbiology ( IF 2.8 ) Pub Date : 2020-06-10 , DOI: 10.1007/s00203-020-01944-6
Ikram Mercha 1, 2 , Nazha Lakram 2 , Mohammed Rachid Kabbour 2 , Mohammed Bouksaim 2 , Fouzia Zkhiri 1 , El Haj El Maadoudi 2
Affiliation  

The objectives of this study were to isolate lactic acid bacteria (LAB) from a raw Moroccan camel milk collected after the incorporation of a specific Argane by-products diet, and to investigate their technological properties as well as their probiotic features. The molecular identification of the isolates indicated that they belong to Weissella confusa, Weissella cibaria or Enterococcus durans species. Our results revealed that the tested isolates have a fast acidifying ability (values ranging between 0.045 ± 0.01 to 0.93 ± 0.01 after only 4 h incubation), important proteolysis, autolysis, lipolytic activities and an important diacetyl and exopolysaccharides production. All these isolates demonstrated a high tolerance to gastrointestinal conditions, namely to gastric simulated juice (survival rate ranged between 75.05 ± 7.88 and 85.55 ± 1.77%) and to bile salts (survival rate between 42.79 ± 1.11 and 82.75 ± 1.01%). The autoaggregation, hydrophobicity and antioxidant activity mean values of the isolates were 13.26–41.16%, 13.23–54.47% and 47.57–63.31%, respectively. Importantly, LAB cultures exhibited antibacterial activity against Gram-negative and Gram-positive pathogenic bacteria and none of the tested isolates presented antibiotic resistance, haemolytic or DNase activities. This study revealed interesting properties for LAB isolated and supported their utilization as autochthone starters for camel milk fermentation that represent a challenge process. These results presented as well the probiotic potential for a possible human consumption.

中文翻译:

从骆驼奶中分离出的肠球菌和魏斯氏菌的益生菌和技术特征,其特征在于阿甘喂养方案

本研究的目的是从加入特定摩洛哥坚果副产品饮食后收集的生摩洛哥骆驼奶中分离乳酸菌 (LAB),并研究它们的技术特性和益生菌特性。分离物的分子鉴定表明它们属于Weissellella confusa、Weissellella cibaria或Enterococcus durans。我们的结果表明,所测试的分离株具有快速酸化能力(仅培养 4 小时后的值范围在 0.045 ± 0.01 至 0.93 ± 0.01 之间)、重要的蛋白水解、自溶、脂解活性和重要的双乙酰和胞外多糖生产。所有这些分离物都表现出对胃肠道疾病的高度耐受性,即胃模拟液(存活率介于 75.05 ± 7.88 和 85.55 ± 1 之间。77%)和胆盐(存活率在 42.79 ± 1.11 和 82.75 ± 1.01% 之间)。分离株的自聚集、疏水性和抗氧化活性平均值分别为 13.26-41.16%、13.23-54.47% 和 47.57-63.31%。重要的是,LAB 培养物表现出对革兰氏阴性和革兰氏阳性病原菌的抗菌活性,并且所有测试的分离株都没有表现出抗生素耐药性、溶血性或 DNase 活性。这项研究揭示了分离出的 LAB 的有趣特性,并支持将其用作代表挑战过程的骆驼奶发酵的自生酮起始剂。这些结果还展示了可能供人类食用的益生菌潜力。分离株的疏水性和抗氧化活性平均值分别为13.26-41.16%、13.23-54.47%和47.57-63.31%。重要的是,LAB 培养物表现出对革兰氏阴性和革兰氏阳性病原菌的抗菌活性,并且所有测试的分离株都没有表现出抗生素耐药性、溶血性或 DNase 活性。这项研究揭示了分离出的 LAB 的有趣特性,并支持将其用作代表挑战过程的骆驼奶发酵的自生酮起始剂。这些结果还展示了可能供人类食用的益生菌潜力。分离株的疏水性和抗氧化活性平均值分别为13.26-41.16%、13.23-54.47%和47.57-63.31%。重要的是,LAB 培养物表现出对革兰氏阴性和革兰氏阳性病原菌的抗菌活性,并且所有测试的分离株都没有表现出抗生素耐药性、溶血性或 DNase 活性。这项研究揭示了分离出的 LAB 的有趣特性,并支持将其用作代表挑战过程的骆驼奶发酵的自生酮起始剂。这些结果还展示了可能供人类食用的益生菌潜力。LAB 培养物表现出对革兰氏阴性和革兰氏阳性病原菌的抗菌活性,并且测试的分离株均未表现出抗生素抗性、溶血性或 DNase 活性。这项研究揭示了分离出的 LAB 的有趣特性,并支持将其用作代表挑战过程的骆驼奶发酵的自生酮起始剂。这些结果还展示了可能供人类食用的益生菌潜力。LAB 培养物表现出对革兰氏阴性和革兰氏阳性病原菌的抗菌活性,并且测试的分离株均未表现出抗生素抗性、溶血性或 DNase 活性。这项研究揭示了分离出的 LAB 的有趣特性,并支持将其用作代表挑战过程的骆驼奶发酵的自生酮起始剂。这些结果还展示了可能供人类食用的益生菌潜力。
更新日期:2020-06-10
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