Although the theoretical foundations of Förster resonance energy transfer (FRET) were laid in the 1940s as part of the quantum physical revolution of the 20th century, it was only in the 1970s that it made its way to biology as a result of the availability of suitable measuring and labeling technologies. Thanks to its ease of application, FRET became widely used for studying molecular associations on the nanometer scale. The development of superresolution techniques at the turn of the millennium promised an unprecedented insight into the structure and function of molecular complexes. Without downplaying the significance of superresolution microscopies this review expresses our view that FRET is still a legitimate tool in the armamentarium of biologists for studying molecular associations since it offers distinct advantages and overcomes certain limitations of superresolution approaches.

中文翻译：

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vadis FRET？超分辨率时代的Förster方法。

尽管作为20世纪量子物理革命的一部分，福斯特共振能量转移（FRET）的理论基础在1940年代奠定了基础，但直到1970年代，由于有合适的适用性，它才进入生物学领域。测量和标签技术。由于其易于使用，FRET被广泛用于研究纳米级的分子缔合。在千年之交，超分辨率技术的发展使人们对分子复合物的结构和功能有了空前的见识。