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Nonomuraea terrae sp. nov., isolated from arid soil
Archives of Microbiology ( IF 2.8 ) Pub Date : 2020-06-10 , DOI: 10.1007/s00203-020-01941-9
Hilal Ay 1
Affiliation  

During the course of isolating rare actinobacteria from unexplored habitats, strain CH32T was obtained from an arid soil sample in eastern Anatolia, Turkey. Polyphasic characterization and comprehensive genome analyses showed that the strain is a member of the genus Nonomuraea and it is closely related to Nonomuraea gerenzanensis ATCC 39727T, Nonomuraea polychroma DSM 43925T and Nonomuraea maritima FXJ7.203T with gene identity level of 98.7%, 98.2% and 98.1%, respectively. The whole-cell hydrolysates contain meso-diaminopimelic acid as diagnostic diaminoacid and glucose, ribose, galactose, mannose and madurose as whole cell sugars. The predominant menaquinones are MK-9(H4), MK-9(H6) and MK-9(H2) while MK-9 exists as minor component. The polar lipid profile consists of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, glycolipid, glycophospholipids, phospholipids and unidentified lipids. The major cellular fatty acids are iso-C16:0 and C17:0 10-methyl. The total genome size is about 9.6 Mb and the G + C content is 71.0%. The genome contains biosynthetic gene clusters encoding for terpenes, siderophores, a type III polyketide synthase, a non-ribosomal polypeptide synthetase and a bacteriocin. The genome-based comparisons of the strain with its phylogenetic neighbours, as indicated by digital DNA–DNA hybridization and average nucleotide identity analyses, reveal that strain CH32T (= JCM 33876T = KCTC 49368T) is a novel member of the genus Nonomuraea, for which Nonomuraea terrae sp. nov. is proposed.

中文翻译:

Nonomuraea terrae sp. 11月,从干旱土壤中分离出来

在从未开发的栖息地中分离稀有放线菌的过程中,从土耳其安纳托利亚东部的干旱土壤样本中获得了 CH32T 菌株。多相表征和综合基因组分析表明,该菌株是野村菌属的一员,与野野村菌ATCC 39727T、多色野野村菌DSM 43925T和野村野村菌FXJ7.203T密切相关,基因同一性水平为98.2%,998.7%, %, 分别。全细胞水解产物含有内消旋二氨基庚二酸作为诊断二氨基酸和葡萄糖、核糖、半乳糖、甘露糖和马杜糖作为全细胞糖。主要的甲基萘醌是 MK-9(H4)、MK-9(H6) 和 MK-9(H2),而 MK-9 作为次要成分存在。极性脂质成分由二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、糖脂、糖磷脂、磷脂和不明脂质。主要的细胞脂肪酸是异 C16:0 和 C17:0 10-甲基。总基因组大小约为 9.6 Mb,G+C 含量为 71.0%。基因组包含编码萜烯、铁载体、III 型聚酮化合物合酶、非核糖体多肽合成酶和细菌素的生物合成基因簇。数字 DNA-DNA 杂交和平均核苷酸同一性分析表明,该菌株与其系统发育邻居的基于基因组的比较表明,菌株 CH32T(= JCM 33876T = KCTC 49368T)是 Nonomuraea 属的新成员,其中Nonomuraea terrae sp. 十一月 被提议。0 10-甲基。总基因组大小约为 9.6 Mb,G+C 含量为 71.0%。基因组包含编码萜烯、铁载体、III 型聚酮化合物合酶、非核糖体多肽合成酶和细菌素的生物合成基因簇。数字 DNA-DNA 杂交和平均核苷酸同一性分析表明,该菌株与其系统发育邻居的基于基因组的比较表明,菌株 CH32T(= JCM 33876T = KCTC 49368T)是 Nonomuraea 属的新成员,其中Nonomuraea terrae sp. 十一月 被提议。0 10-甲基。总基因组大小约为 9.6 Mb,G+C 含量为 71.0%。基因组包含编码萜烯、铁载体、III 型聚酮化合物合酶、非核糖体多肽合成酶和细菌素的生物合成基因簇。数字 DNA-DNA 杂交和平均核苷酸同一性分析表明,该菌株与其系统发育邻居的基于基因组的比较表明,菌株 CH32T(= JCM 33876T = KCTC 49368T)是 Nonomuraea 属的新成员,其中Nonomuraea terrae sp. 十一月 被提议。数字 DNA-DNA 杂交和平均核苷酸同一性分析表明,该菌株与其系统发育邻居的基于基因组的比较表明,菌株 CH32T(= JCM 33876T = KCTC 49368T)是 Nonomuraea 属的新成员,其中Nonomuraea terrae sp. 十一月 被提议。数字 DNA-DNA 杂交和平均核苷酸同一性分析表明,该菌株与其系统发育邻居的基于基因组的比较表明,菌株 CH32T(= JCM 33876T = KCTC 49368T)是 Nonomuraea 属的新成员,其中Nonomuraea terrae sp. 十一月 被提议。
更新日期:2020-06-10
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