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X-Ray-Based Irradiation of Larvae and Pupae of the Navel Orangeworm (Lepidoptera: Pyralidae)
Journal of Economic Entomology ( IF 2.2 ) Pub Date : 2020-06-19 , DOI: 10.1093/jee/toaa111
Ronald Haff 1 , Inna Ovchinnikova 1 , Peishih Liang 1 , Noreen Mahoney 1 , Wai Gee 1 , Joseph Gomez 1 , Natsuko Toyofuku 1 , Eric Jackson 1 , Robert Hnasko 1 , Douglas Light 1
Affiliation  

Abstract The suitability of adult male the navel orangeworm, Amyelois transitella (Walker) for Sterile InsectTechnique (SIT) has been reported for both high energy gamma (>1 MeV) and low energy x-ray (90 keV) sterilization. However, research regarding sterilization of NOW larvae and pupae by gamma irradiation indicated nonsuitability due to high mortality. Here, NOW larvae and pupae were irradiated to doses up to 50 Gy with 90 keV x-rays, then paired with nonirradiated colony mates. Sterility of surviving insects was determined by the presence or absence of hatched neonates. While presence of offspring does not guarantee viability, the absence does guarantee sterility (as is appropriate for SIT) and was thus the measure used here. Early stage larvae experienced 77% mortality at a dose of 30 Gy, versus 20% for nonirradiated control. At 40 Gy, mortality reached 98%. Of surviving early stage larvae at 30 Gy, 29% of moth pairs produced offspring. For late stage larvae, no offspring were produced at 40 Gy, but mortality was 73%. For pupae, mortality reached 53% at 30 Gy with 13% still producing neonates, while mortality reached 98% at 40 Gy. These results are consistent with reported results for gamma irradiation of NOW larvae where sterility was observed somewhere between the 30 Gy and 60 Gy data points, but mortality was high. This further confirms the lack of suitability of NOW irradiated in the larval stage, whether by gamma or x-ray, and supports the hypothesis that x-ray and gamma treatments are biologically equivalent at equal doses.

中文翻译:

脐橙虫幼虫和蛹的 X 射线辐照(鳞翅目:Pyralidae)

摘要 据报道,成年雄性脐橙蠕虫 Amyelois transella (Walker) 适用于不育昆虫技术 (SIT) 的高能伽马 (>1 MeV) 和低能 X 射线 (90 keV) 灭菌。然而,关于通过伽马辐射对 NOW 幼虫和蛹进行绝育的研究表明,由于高死亡率,不适用。在这里,NOW 幼虫和蛹接受了高达 50 Gy 的 90 keV X 射线照射,然后与未受照射的菌落配偶配对。存活昆虫的不育性由孵化的新生儿的存在与否决定。虽然后代的存在并不能保证生存能力,但不存在确实保证了不育(这对于 SIT 是合适的),因此是这里使用的措施。在 30 Gy 的剂量下,早期幼虫的死亡率为 77%,而未辐照对照组的死亡率为 20%。在 40 Gy 时,死亡率达到98%。在 30 Gy 下存活的早期幼虫中,29% 的蛾对产生了后代。对于晚期幼虫,在 40 Gy 下没有产生后代,但死亡率为 73%。对于蛹,在 30 Gy 时死亡率达到 53%,其中 13% 仍在生产新生儿,而在 40 Gy 时死亡率达到 98%。这些结果与 NOW 幼虫的伽马辐射报告结果一致,在 30 Gy 和 60 Gy 数据点之间观察到不育,但死亡率很高。这进一步证实了 NOW 在幼虫阶段缺乏适用性,无论是通过伽马还是 X 射线,并支持 X 射线和伽马治疗在同等剂量下在生物学上是等效的假设。在 40 Gy 下没有产生后代,但死亡率为 73%。对于蛹,在 30 Gy 时死亡率达到 53%,其中 13% 仍在生产新生儿,而在 40 Gy 时死亡率达到 98%。这些结果与 NOW 幼虫的伽马辐射报告结果一致,在 30 Gy 和 60 Gy 数据点之间观察到不育,但死亡率很高。这进一步证实了 NOW 在幼虫阶段缺乏适用性,无论是通过伽马还是 X 射线,并支持 X 射线和伽马治疗在同等剂量下在生物学上是等效的假设。在 40 Gy 下没有产生后代,但死亡率为 73%。对于蛹,在 30 Gy 时死亡率达到 53%,其中 13% 仍在生产新生儿,而在 40 Gy 时死亡率达到 98%。这些结果与 NOW 幼虫的伽马辐射报告结果一致,在 30 Gy 和 60 Gy 数据点之间观察到不育,但死亡率很高。这进一步证实了 NOW 在幼虫阶段缺乏适用性,无论是通过伽马还是 X 射线,并支持 X 射线和伽马治疗在同等剂量下在生物学上是等效的假设。这些结果与 NOW 幼虫的伽马辐射报告结果一致,在 30 Gy 和 60 Gy 数据点之间观察到不育,但死亡率很高。这进一步证实了 NOW 在幼虫阶段缺乏适用性,无论是通过伽马还是 X 射线,并支持 X 射线和伽马治疗在同等剂量下在生物学上是等效的假设。这些结果与 NOW 幼虫的伽马辐射报告结果一致,在 30 Gy 和 60 Gy 数据点之间观察到不育,但死亡率很高。这进一步证实了 NOW 在幼虫阶段缺乏适用性,无论是通过伽马还是 X 射线,并支持 X 射线和伽马治疗在同等剂量下在生物学上是等效的假设。
更新日期:2020-06-19
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