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Carbon source utilization patterns in dental plaque and microbial responses to sucrose, lactose, and phenylalanine consumption in severe early childhood caries.
Journal of Oral Microbiology ( IF 4.5 ) Pub Date : 2020-06-23 , DOI: 10.1080/20002297.2020.1782696
Weihua Shi 1 , Jing Tian 1 , He Xu 1 , Guiyan Wang 1 , Qiong Zhou 1 , Man Qin 1
Affiliation  

ABSTRACT

Background

Severe early childhood caries (S-ECC) is mainly caused by the interaction of microbiota and environmental factors. However, the metabolic profiles of S-ECC microbial communities and the community-level microbial responses to carbohydrates and amino acids are poorly understood.

Methods

We collected supragingival plaques from 15 caries-free (CF) and 14 S-ECC children. Cultivation on Biolog AN microplates together with next-generation sequencing was used to analyze sole carbon source utilization patterns and microbial responses to sucrose, lactose and phenylalanine.

Results

S-ECC plaques had greater overall metabolic activity than those of CF ones. Comparing with CF, S-ECC plaques utilized more sucrose and lactose but less phenylalanine and then had greater response to carbohydrates. A remarkable increase of non-mutans Streptococci was observed in sucrose and lactose consumption. Lactose led to less differently distributed taxa than sucrose in both CF and S-ECC groups. Sucrose made the originally different S-ECC and CF communities eventually became similar to each other, but they remained dissimilar in lactose.

Conclusion

S-ECC plaques had more active interaction with cariogenic carbohydrates like sucrose and lactose than healthy plaques. We supported lactose has less cariogenicity compared with sucrose from microbial community structural aspect. Phenylalanine may have a potentially inhibitory effect on caries development.



中文翻译:

严重儿童早期龋齿中牙菌斑中的碳源利用模式和微生物对蔗糖、乳糖和苯丙氨酸消耗的反应。

摘要

背景

严重幼儿龋齿(S-ECC)主要是由微生物群和环境因素的相互作用引起的。然而,人们对 S-ECC 微生物群落的代谢特征以及群落水平的微生物对碳水化合物和氨基酸的反应知之甚少。

方法

我们收集了 15 名无龋 (CF) 和 14 名 S-ECC 儿童的龈上斑块。Biolog AN 微孔板培养与下一代测序一起用于分析唯一碳源利用模式和微生物对蔗糖、乳糖和苯丙氨酸的反应。

结果

S-ECC 斑块的整体代谢活性高于 CF 斑块。与CF相比,S-ECC斑块利用更多的蔗糖和乳糖,但苯丙氨酸更少,对碳水化合物的反应更大。在蔗糖和乳糖消耗中观察到非变形链球菌显着增加。在 CF 和 S-ECC 组中,与蔗糖相比,乳糖的分布差异较小。蔗糖使原本不同的 S-ECC 和 CF 群落最终变得相似,但它们的乳糖仍然不同。

结论

S-ECC 斑块与蔗糖和乳糖等致龋碳水化合物的相互作用比健康斑块更活跃。从微生物群落结构方面,我们支持乳糖与蔗糖相比具有较少的致龋性。苯丙氨酸可能对龋齿的发展具有潜在的抑制作用。

更新日期:2020-06-23
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