Anther development and pollen tube elongation are key steps for pollination and fertilization. The timing and spatial distribution of reactive oxygen species (ROS) and programmed cell death are central to these processes, but the regulatory mechanism of ROS production is not well understood. Inflorescence deficient in abscission (IDA) is implicated in many plant development and responses to environmental stimuli. However, their role in reproductive development is still unknown. We generated tomato knockout lines (CR‐slida) of an IDA homolog (SlIDA), which is expressed in the tapetum, septum and pollen tube, and observed a severe defect in male gametes. Further analysis indicated that there was a programmed cell death defect in the tapetum and septum and a failure of anther dehiscence in the CR‐slida lines, likely related to insufficient ROS signal. Liquid chromatography‐tandem mass spectrometry identified mature SlIDA as a 14‐mer EPIP peptide, which was shown to be secreted, and a complementation experiment showed that application of a synthetic 14‐mer EPIP peptide rescued the CR‐slida defect and enhanced the ROS signal. Moreover, the application of the ROS scavengers diphenyleneiodonium or Mn‐TMPP suppressed peptide function. Collectively, our results revealed that SlIDA plays an essential role in pollen development and pollen tube elongation by modulating ROS homeostasis.

中文翻译：

---

番茄SlIDA通过改变活性氧的稳态来在番茄施肥中发挥关键作用。

花药发育和花粉管伸长是授粉和施肥的关键步骤。活性氧（ROS）的时间和空间分布以及程序性细胞死亡是这些过程的关键，但对ROS产生的调节机制尚不甚了解。脱落（IDA）缺乏的花序与许多植物的发育和对环境刺激的反应有关。但是，它们在生殖发育中的作用仍然未知。我们生成了IDA同源物（SlIDA）的番茄敲除品系（CR‐slida）在绒毡层，隔膜和花粉管中表达，并观察到雄配子严重缺陷。进一步的分析表明，绒毡层和隔膜中存在程序性的细胞死亡缺陷，而CR-slida系中的花药开裂失败，可能与ROS信号不足有关。液相色谱-串联质谱鉴定出成熟的SlIDA为14-mer EPIP肽，该肽已被分泌，补充实验表明，使用合成的14-mer EPIP肽可挽救CR-slida缺陷并增强ROS信号。此外，ROS清除剂二苯撑碘鎓或Mn-TMPP的应用抑制了肽的功能。总的来说，我们的结果表明SlIDA 通过调节ROS稳态在花粉发育和花粉管伸长中起重要作用。